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Article

MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

1
The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
2
The Seventh Class of 2012 year entry, the Third Clinical College, Chongqing Medical University, Chongqing 400016, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Christophe Nicot
Int. J. Mol. Sci. 2015, 16(11), 27945-27955; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms161126075
Received: 24 October 2015 / Revised: 13 November 2015 / Accepted: 16 November 2015 / Published: 24 November 2015
(This article belongs to the Special Issue MicroRNA Regulation)
Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3′UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7. View Full-Text
Keywords: kidney fibrosis; miR542-3p; bone morphogenetic protein 7 (BMP7); Epithelial-Eesenchymal Transition (EMT); transforming growth factor beta 1 (TGFβ1) kidney fibrosis; miR542-3p; bone morphogenetic protein 7 (BMP7); Epithelial-Eesenchymal Transition (EMT); transforming growth factor beta 1 (TGFβ1)
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MDPI and ACS Style

Liu, Z.; Zhou, Y.; Yuan, Y.; Nie, F.; Peng, R.; Li, Q.; Lyu, Z.; Mao, Z.; Huang, L.; Zhou, L.; Li, Y.; Hao, J.; Ni, D.; Jin, Q.; Long, Y.; Ju, P.; Yu, W.; Liu, J.; Hu, Y.; Zhou, Q. MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e. Int. J. Mol. Sci. 2015, 16, 27945-27955. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms161126075

AMA Style

Liu Z, Zhou Y, Yuan Y, Nie F, Peng R, Li Q, Lyu Z, Mao Z, Huang L, Zhou L, Li Y, Hao J, Ni D, Jin Q, Long Y, Ju P, Yu W, Liu J, Hu Y, Zhou Q. MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e. International Journal of Molecular Sciences. 2015; 16(11):27945-27955. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms161126075

Chicago/Turabian Style

Liu, Zhicheng, Yuru Zhou, Yue Yuan, Fang Nie, Rui Peng, Qianyin Li, Zhongshi Lyu, Zhaomin Mao, Liyuan Huang, Li Zhou, Yiman Li, Jing Hao, Dongsheng Ni, Qianni Jin, Yaoshui Long, Pan Ju, Wen Yu, Jianing Liu, Yanxia Hu, and Qin Zhou. 2015. "MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e" International Journal of Molecular Sciences 16, no. 11: 27945-27955. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms161126075

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