Next Article in Journal
Wnt Signaling in Leukemia and Its Bone Marrow Microenvironment
Next Article in Special Issue
The Role of Losartan as a Potential Neuroregenerative Pharmacological Agent after Aneurysmal Subarachnoid Haemorrhage
Previous Article in Journal
Investigation of a Staphylococcus argenteus Strain Involved in a Chronic Prosthetic-Joint Infection
Previous Article in Special Issue
Epigenetic Mechanisms Underlying Adult Post Stroke Neurogenesis
Article

Neurodegeneration, Myelin Loss and Glial Response in the Three-Vessel Global Ischemia Model in Rat

1
Laboratory of Neurobiology, Research Institute of Biology and Biophysics, Tomsk State University, Lenina Ave., 634050 Tomsk, Russia
2
Laboratory of Pharmacology of Blood Circulation, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Lenina Ave., 634028 Tomsk, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6246; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176246
Received: 17 July 2020 / Revised: 21 August 2020 / Accepted: 26 August 2020 / Published: 28 August 2020
(This article belongs to the Special Issue Neuroregeneration and Brain Repair after Stroke)
(1) Background: Although myelin disruption is an integral part of ischemic brain injury, it is rarely the subject of research, particularly in animal models. This study assessed for the first time, myelin and oligodendrocyte loss in a three-vessel model of global cerebral ischemia (GCI), which causes hippocampal damage. In addition, we investigated the relationships between demyelination and changes in microglia and astrocytes, as well as oligodendrogenesis in the hippocampus; (2) Methods: Adult male Wistar rats (n = 15) underwent complete interruption of cerebral blood flow for 7 min by ligation of the major arteries supplying the brain or sham-operation. At 10 and 30 days after the surgery, brain slices were stained for neurodegeneration with Fluoro-Jade C and immunohistochemically to assess myelin content (MBP+ percentage of total area), oligodendrocyte (CNP+ cells) and neuronal (NeuN+ cells) loss, neuroinflammation (Iba1+ cells), astrogliosis (GFAP+ cells) and oligodendrogenesis (NG2+ cells); (3) Results: 10 days after GCI significant myelin and oligodendrocyte loss was found only in the stratum oriens and stratum pyramidale. By the 30th day, demyelination in these hippocampal layers intensified and affected the substratum radiatum. In addition to myelin damage, activation and an increase in the number of microglia and astrocytes in the corresponding layers, a loss of the CA1 pyramidal neurons, and neurodegeneration in the neocortex and thalamus was observed. At a 10-day time point, we observed rod-shaped microglia in the substratum radiatum. Parallel with ongoing myelin loss on the 30th day after ischemia, we found significant oligodendrogenesis in demyelinated hippocampal layers; (4) Conclusions: Our study showed that GCI-simulating cardiac arrest in humans—causes not only the loss of pyramidal neurons in the CA1 field, but also the myelin loss of adjacent layers of the hippocampus. View Full-Text
Keywords: global cerebral ischemia; GCI; hippocampus; CA1; neurodegeneration; myelin; oligodendrocyte; inflammation; rod-shaped microglia; oligodendrogenesis global cerebral ischemia; GCI; hippocampus; CA1; neurodegeneration; myelin; oligodendrocyte; inflammation; rod-shaped microglia; oligodendrogenesis
Show Figures

Figure 1

MDPI and ACS Style

Anan’ina, T.; Kisel, A.; Kudabaeva, M.; Chernysheva, G.; Smolyakova, V.; Usov, K.; Krutenkova, E.; Plotnikov, M.; Khodanovich, M. Neurodegeneration, Myelin Loss and Glial Response in the Three-Vessel Global Ischemia Model in Rat. Int. J. Mol. Sci. 2020, 21, 6246. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176246

AMA Style

Anan’ina T, Kisel A, Kudabaeva M, Chernysheva G, Smolyakova V, Usov K, Krutenkova E, Plotnikov M, Khodanovich M. Neurodegeneration, Myelin Loss and Glial Response in the Three-Vessel Global Ischemia Model in Rat. International Journal of Molecular Sciences. 2020; 21(17):6246. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176246

Chicago/Turabian Style

Anan’ina, Tatiana, Alena Kisel, Marina Kudabaeva, Galina Chernysheva, Vera Smolyakova, Konstantin Usov, Elena Krutenkova, Mark Plotnikov, and Marina Khodanovich. 2020. "Neurodegeneration, Myelin Loss and Glial Response in the Three-Vessel Global Ischemia Model in Rat" International Journal of Molecular Sciences 21, no. 17: 6246. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176246

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop