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Article

Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem (iPS) Cells Suppress or Activate T Cells via Costimulatory Signals

1
Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku Kobe 650-0047, Japan
2
Department of Ophthalmology, Kobe City Eye Hospital, 2-1-8 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan
3
Department of Ophthalmology and Visual Science, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6507; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186507
Received: 18 August 2020 / Revised: 31 August 2020 / Accepted: 1 September 2020 / Published: 5 September 2020
Human retinal pigment epithelial (RPE) cells derived from induced pluripotent stem (iPS) cells have immunosuppressive properties. However, RPE cells are also known as immunogenic cells, and they have major histocompatibility complex expression and produce inflammatory proteins, and thus experience immune rejection after transplantation. In this study, to confirm the immunological properties of IPS-RPE cells, we examined whether human RPE cells derived from iPS cells could suppress or stimulate inflammatory T cells from uveitis patients via costimulatory signals. We established T cells from patients with active uveitis as target cells and used iPS-RPE cells as effector cells. As a result, cultured iPS-RPE cells inhibited cell proliferation and the production of IFN-γ by activated uveitis CD4+ T cells, especially Th1-type T cells. In contrast, iPS-RPE cells stimulated T cells of uveitis patients. The iPS-RPE cells constitutively expressed B7-H1/CD274 and B7-DC/CD273, and suppressed the activation of T cells via the PD-1 receptor. iPS-RPE expressed these negative costimulatory molecules, especially when RPE cells were pretreated with recombinant IFN-γ. In addition, iPS-RPE cells also expressed B7-H3/CD276 costimulatory molecules and activated uveitis T cells through the B7-H3-TLT-2 receptor. Thus, cultured iPS-derived retinal cells can suppress or activate inflammatory T cells in vitro through costimulatory interactions. View Full-Text
Keywords: iPS cells; retinal pigment epithelial cells; uveitis; suppression; stimulation; costimulatory molecules iPS cells; retinal pigment epithelial cells; uveitis; suppression; stimulation; costimulatory molecules
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MDPI and ACS Style

Sugita, S.; Futatsugi, Y.; Ishida, M.; Edo, A.; Takahashi, M. Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem (iPS) Cells Suppress or Activate T Cells via Costimulatory Signals. Int. J. Mol. Sci. 2020, 21, 6507. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186507

AMA Style

Sugita S, Futatsugi Y, Ishida M, Edo A, Takahashi M. Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem (iPS) Cells Suppress or Activate T Cells via Costimulatory Signals. International Journal of Molecular Sciences. 2020; 21(18):6507. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186507

Chicago/Turabian Style

Sugita, Sunao, Yoko Futatsugi, Masaaki Ishida, Ayaka Edo, and Masayo Takahashi. 2020. "Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem (iPS) Cells Suppress or Activate T Cells via Costimulatory Signals" International Journal of Molecular Sciences 21, no. 18: 6507. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186507

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