Molecular Interaction of Protein-Pigment C-Phycocyanin with Bovine Serum Albumin in a Gomphosis Structure Inhibiting Amyloid Formation
Abstract
:1. Introduction
2. Results
2.1. Inhibitory Effect of C-phycocyanin on Formation of Amyloid Fibrils
2.2. Fluorescence Intensity and Kinetic Fitting Results
2.3. CD Spectra
2.4. Molecular Docking
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Inhibitory Effect of BSA on Amyloid Formation In Vitro
4.3. Kinetic Analysis
4.4. Circular Dichroism Analysis
4.5. Molecular Docking
4.6. Data Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
BSA | Bovine serum albumin |
CD | Circular dichroism |
ThT | Thioflavine T |
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C-phycocyanin (µg/mL) | k1 | k2 | R2 | Half-Life (t1/2, Days) |
---|---|---|---|---|
0.0 | 1.820 × 10−2 ± 3.14 × 10−3 | 4.198 × 10−5 ± 2.68 × 10−5 | 0.9931 | 12.43 |
0.5 | 1.445 × 10−2 ± 2.07 × 10−3 | 4.003 × 10−5 ± 4.28 × 10−6 | 0.9945 | 14.41 |
2.5 | 1.256 × 10−2 ± 3.42 × 10−3 | 4.894 × 10−5 ± 8.83 × 10−6 | 0.9804 | 13.89 |
5.0 | 1.247 × 10−2 ± 1.77 × 10−4 | 4.444 × 10−5 ± 4.63 × 10−6 | 0.9939 | 14.69 |
25.0 | 5.610 × 10−3 ± 9.42 × 10−4 | 5.930 × 10−5 ± 5.04 × 10−6 | 0.9928 | 16.45 |
50.0 | 2.620 × 10−3 ± 3.03 × 10−4 | 7.012 × 10−5 ± 3.34 × 10−6 | 0.9969 | 17.73 |
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Luo, Y.-C.; Jing, P. Molecular Interaction of Protein-Pigment C-Phycocyanin with Bovine Serum Albumin in a Gomphosis Structure Inhibiting Amyloid Formation. Int. J. Mol. Sci. 2020, 21, 8207. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218207
Luo Y-C, Jing P. Molecular Interaction of Protein-Pigment C-Phycocyanin with Bovine Serum Albumin in a Gomphosis Structure Inhibiting Amyloid Formation. International Journal of Molecular Sciences. 2020; 21(21):8207. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218207
Chicago/Turabian StyleLuo, Yi-Cong, and Pu Jing. 2020. "Molecular Interaction of Protein-Pigment C-Phycocyanin with Bovine Serum Albumin in a Gomphosis Structure Inhibiting Amyloid Formation" International Journal of Molecular Sciences 21, no. 21: 8207. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218207