Transcription Factor PLAGL1 Is Associated with Angiogenic Gene Expression in the Placenta
Abstract
:1. Introduction
2. Results
2.1. E9.5-Specific Genes and Enhancers Are Associated with Vasculature Development
2.2. Plagl1 Is Highly Expressed in the e9.5 Placenta and the PLAGL1 Binding Motif Is Enriched in e9.5-Specific Enhancer Regions
2.3. Plagl1 Is Expressed in Endothelial Cells of the Labyrinthine Layer
2.4. Plagl1 Is Associated with Blood Vessel Development and Insulin Response
2.5. PLAGL1 Knockdown in the HTR-8/SVneo Cell Line Predicts a Role in Blood Vessel Remodeling
2.6. Relationship between Mouse and Human Plagl1 Results
3. Discussions
4. Materials and Methods
4.1. Filtering of e7.5 and e9.5 Placenta RNA-seq Data
4.2. Binding Site Predictions
4.3. Plagl1 Timepoint Expression qPCR
4.4. RNA-Scope
4.5. Immunohistochemistry Staining
4.6. GDM Placenta qPCR
4.7. TissueEnrich Analysis
4.8. Human Protein Atlas Placenta Analysis
4.9. siRNA knockdown and qPCR
4.10. HTR-8/SVneo RNA-seq
4.11. Tube Formation Assay
4.12. Cloning
4.13. Enhancer Testing with a Dual Glow Luciferase Assay
4.14. Ontology Analysis
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
e | Embryonic day |
TF | Transcription factor |
PLAGL1 | pleiomorphic adenoma gene-like 1 |
GDM | gestational diabetes mellitus |
FPKM | fragments per kilobase of transcript per million of mapped reads |
TPM | transcript per million |
GREAT | Genomic Regions Enrichment of Annotations Tool |
Ctrl | Control diet |
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Starks, R.R.; Abu Alhasan, R.; Kaur, H.; Pennington, K.A.; Schulz, L.C.; Tuteja, G. Transcription Factor PLAGL1 Is Associated with Angiogenic Gene Expression in the Placenta. Int. J. Mol. Sci. 2020, 21, 8317. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218317
Starks RR, Abu Alhasan R, Kaur H, Pennington KA, Schulz LC, Tuteja G. Transcription Factor PLAGL1 Is Associated with Angiogenic Gene Expression in the Placenta. International Journal of Molecular Sciences. 2020; 21(21):8317. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218317
Chicago/Turabian StyleStarks, Rebekah R., Rabab Abu Alhasan, Haninder Kaur, Kathleen A. Pennington, Laura C. Schulz, and Geetu Tuteja. 2020. "Transcription Factor PLAGL1 Is Associated with Angiogenic Gene Expression in the Placenta" International Journal of Molecular Sciences 21, no. 21: 8317. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218317