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Vaginal Lactobacilli and Vaginal Dysbiosis-Associated Bacteria Differently Affect Cervical Epithelial and Immune Homeostasis and Anti-Viral Defenses

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Department of Experimental and Clinical Medicine, University of Firenze, 50134 Florence, Italy
2
Clinical Microbiology and Virology Unit, Careggi University Hospital, 50139 Florence, Italy
3
Department of Neurosciences, Psychology, Drug Research and Child Health, Section of Pharmaceutical and Nutraceutical Sciences, University of Firenze, 50134 Firenze, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Rosa Sessa
Int. J. Mol. Sci. 2021, 22(12), 6487; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22126487
Received: 16 April 2021 / Revised: 7 June 2021 / Accepted: 14 June 2021 / Published: 17 June 2021
Persistent infection with High Risk-Human Papilloma Viruses (HR-HPVs) is a primary cause of cervical cancer worldwide. Vaginal-dysbiosis-associated bacteria were correlated with the persistence of HR-HPVs infection and with increased cancer risk. We obtained strains of the most represented bacterial species in vaginal microbiota and evaluated their effects on the survival of cervical epithelial cells and immune homeostasis. The contribution of each species to supporting the antiviral response was also studied. Epithelial cell viability was affected by culture supernatants of most vaginal-dysbiosis bacteria, whereas Lactobacillus gasseri or Lactobacillus jensenii resulted in the best stimulus to induce interferon-γ (IFN-γ) production by human mononuclear cells from peripheral blood (PBMCs). Although vaginal-dysbiosis-associated bacteria induced the IFN-γ production, they were also optimal stimuli to interleukin-17 (IL-17) production. A positive correlation between IL-17 and IFN-γ secretion was observed in cultures of PBMCs with all vaginal-dysbiosis-associated bacteria suggesting that the adaptive immune response induced by these strains is not dominated by TH1 differentiation with reduced availability of IFN-γ, cytokine most effective in supporting virus clearance. Based on these results, we suggest that a vaginal microbiota dominated by lactobacilli, especially by L. gasseri or L. jensenii, may be able to assist immune cells with clearing HPV infection, bypasses the viral escape and restores immune homeostasis. View Full-Text
Keywords: vaginal microbiota; Lactobacillus; Gardnerella vaginalis; IFN-γ; IL-17 vaginal microbiota; Lactobacillus; Gardnerella vaginalis; IFN-γ; IL-17
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MDPI and ACS Style

Nicolò, S.; Tanturli, M.; Mattiuz, G.; Antonelli, A.; Baccani, I.; Bonaiuto, C.; Baldi, S.; Nannini, G.; Menicatti, M.; Bartolucci, G.; Rossolini, G.M.; Amedei, A.; Torcia, M.G. Vaginal Lactobacilli and Vaginal Dysbiosis-Associated Bacteria Differently Affect Cervical Epithelial and Immune Homeostasis and Anti-Viral Defenses. Int. J. Mol. Sci. 2021, 22, 6487. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22126487

AMA Style

Nicolò S, Tanturli M, Mattiuz G, Antonelli A, Baccani I, Bonaiuto C, Baldi S, Nannini G, Menicatti M, Bartolucci G, Rossolini GM, Amedei A, Torcia MG. Vaginal Lactobacilli and Vaginal Dysbiosis-Associated Bacteria Differently Affect Cervical Epithelial and Immune Homeostasis and Anti-Viral Defenses. International Journal of Molecular Sciences. 2021; 22(12):6487. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22126487

Chicago/Turabian Style

Nicolò, Sabrina, Michele Tanturli, Giorgio Mattiuz, Alberto Antonelli, Ilaria Baccani, Chiara Bonaiuto, Simone Baldi, Giulia Nannini, Marta Menicatti, Gianluca Bartolucci, Gian M. Rossolini, Amedeo Amedei, and Maria G. Torcia. 2021. "Vaginal Lactobacilli and Vaginal Dysbiosis-Associated Bacteria Differently Affect Cervical Epithelial and Immune Homeostasis and Anti-Viral Defenses" International Journal of Molecular Sciences 22, no. 12: 6487. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22126487

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