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Article

A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells

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Department of Pharmacology, Pharmacognosy and Botany, Complutense University, 28040 Madrid, Spain
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SALURIS, 28040 Madrid, Spain
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UCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4099-002 Porto, Portugal
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School of Sport and Exercise Sciences, University of Thessaly, Karies, 42100 Trikala, Greece
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Faculty of Education, Health and Wellbeing, Wolverhampton University, Walsall WV1 1LY, UK
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BME, Biomechanical Solutions, 43150 Karditsa, Greece
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Department of Exercise Science, Chatham University, Pittsburgh, PA 15232, USA
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Move-Cor Inc., Pittsburgh, PA 15017, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Alberto Falchetti
Int. J. Mol. Sci. 2021, 22(19), 10195; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221910195
Received: 31 August 2021 / Revised: 17 September 2021 / Accepted: 17 September 2021 / Published: 22 September 2021
(This article belongs to the Special Issue Secondary Osteoporosis in Adults 2.0)
Glucocorticoid-induced osteoporosis (GIO) is one of the most common secondary forms of osteoporosis. GIO is partially due to the apoptosis of osteoblasts and osteocytes. In addition, high doses of dexamethasone (DEX), a synthetic glucocorticoid receptor agonist, induces neurodegeneration by initiating inflammatory processes leading to neural apoptosis. Here, a neuroprotective bovine colostrum against glucocorticoid-induced neuronal damage was investigated for its anti-apoptotic activity in glucocorticoid-treated MC3T3-E1 osteoblastic cells. A model of apoptotic osteoblastic cells was developed by exposing MC3T3-E1 cells to DEX (0–700 μM). Colostrum co-treated with DEX was executed at 0.1–5.0 mg/mL. Cell viability was measured for all treatment schedules. Caspase-3 activation was assessed to determine both osteoblast apoptosis under DEX exposure and its potential prevention by colostrum co-treatment. Glutathione reduced (GSH) was measured to determine whether DEX-mediated oxidative stress-driven apoptosis is alleviated by colostrum co-treatment. Western blot was performed to determine the levels of p-ERK1/2, Bcl-XL, Bax, and Hsp70 proteins upon DEX or DEX plus colostrum exposure. Colostrum prevented the decrease in cell viability and the increase in caspase-3 activation and oxidative stress caused by DEX exposure. Cells, upon colostrum co-treated with DEX, exhibited higher levels of p-ERK1/2 and lower levels of Bcl-XL, Bax, and Hsp70. Our data support the notion that colostrum may be able to reduce DEX-induced apoptosis possibly via the activation of the ERK pathway and modulation of the Hsp70 system. We provided preliminary evidence on how bovine colostrum, as a complex and multi-component dairy product, in addition to its neuroprotective action, may affect osteoblastic cell survival undergoing apoptosis. View Full-Text
Keywords: bovine colostrum; neuroprotective; osteoblast; glucocorticoid-induced osteoporosis; dexamethasone; ERK1/2; caspase-3; glutathione reduced; Hsp70 bovine colostrum; neuroprotective; osteoblast; glucocorticoid-induced osteoporosis; dexamethasone; ERK1/2; caspase-3; glutathione reduced; Hsp70
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MDPI and ACS Style

Martin-Aragon, S.; Bermejo-Bescós, P.; Benedí, J.; Raposo, C.; Marques, F.; Kydonaki, E.K.; Gkiata, P.; Koutedakis, Y.; Ntina, G.; Carrillo, A.E.; Amorim, T. A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells. Int. J. Mol. Sci. 2021, 22, 10195. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221910195

AMA Style

Martin-Aragon S, Bermejo-Bescós P, Benedí J, Raposo C, Marques F, Kydonaki EK, Gkiata P, Koutedakis Y, Ntina G, Carrillo AE, Amorim T. A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells. International Journal of Molecular Sciences. 2021; 22(19):10195. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221910195

Chicago/Turabian Style

Martin-Aragon, Sagrario, Paloma Bermejo-Bescós, Juana Benedí, Carlos Raposo, Franklim Marques, Eirini K. Kydonaki, Paraskevi Gkiata, Yiannis Koutedakis, Georgia Ntina, Andres E. Carrillo, and Tânia Amorim. 2021. "A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells" International Journal of Molecular Sciences 22, no. 19: 10195. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221910195

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