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Time-Lapse Flow Cytometry: A Robust Tool to Assess Physiological Parameters Related to the Fertilizing Capability of Human Sperm
Open AccessReview

Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals

1
Department of Molecular Biology, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 11600 Montevideo, Uruguay
2
Biochemistry-Molecular Biology, Facultad de Ciencias, Universidad de la República (UdelaR), 11400 Montevideo, Uruguay
*
Authors to whom correspondence should be addressed.
Academic Editor: Stefano Papa
Int. J. Mol. Sci. 2021, 22(3), 1151; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031151
Received: 8 December 2020 / Revised: 12 January 2021 / Accepted: 17 January 2021 / Published: 25 January 2021
Mammalian testes are very heterogeneous organs, with a high number of different cell types. Testicular heterogeneity, together with the lack of reliable in vitro culture systems of spermatogenic cells, have been an obstacle for the characterization of the molecular bases of the unique events that take place along the different spermatogenic stages. In this context, flow cytometry has become an invaluable tool for the analysis of testicular heterogeneity, and for the purification of stage-specific spermatogenic cell populations, both for basic research and for clinical applications. In this review, we highlight the importance of flow cytometry for the advances on the knowledge of the molecular groundwork of spermatogenesis in mammals. Moreover, we provide examples of different approaches to the study of spermatogenesis that have benefited from flow cytometry, including the characterization of mutant phenotypes, transcriptomics, epigenetic and genome-wide chromatin studies, and the attempts to establish cell culture systems for research and/or clinical aims such as infertility treatment. View Full-Text
Keywords: spermatogenesis; flow cytometry; FACS; male infertility spermatogenesis; flow cytometry; FACS; male infertility
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MDPI and ACS Style

Rodríguez-Casuriaga, R.; Geisinger, A. Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals. Int. J. Mol. Sci. 2021, 22, 1151. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031151

AMA Style

Rodríguez-Casuriaga R, Geisinger A. Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals. International Journal of Molecular Sciences. 2021; 22(3):1151. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031151

Chicago/Turabian Style

Rodríguez-Casuriaga, Rosana; Geisinger, Adriana. 2021. "Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals" Int. J. Mol. Sci. 22, no. 3: 1151. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031151

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