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Discovery of a Small Molecule Inhibitor of Human Adenovirus Capable of Preventing Escape from the Endosome
Open AccessArticle

Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus

1
Department of Experimental Pharmacology, Mossakowski Medical Research Institute, Polish Academy of Sciences, Pawinskiego 5, 02-106 Warsaw, Poland
2
Chair and Department of Medical Microbiology, Medical University of Warsaw, 02-091 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Kamalendra Singh
Int. J. Mol. Sci. 2021, 22(4), 2020; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042020
Received: 4 January 2021 / Revised: 4 February 2021 / Accepted: 15 February 2021 / Published: 18 February 2021
(This article belongs to the Special Issue Virus Replication)
Adenovirus infections tend to be mild, but they may pose a serious threat for young and immunocompromised individuals. The treatment is complicated because there are no approved safe and specific drugs for adenovirus infections. Here, we present evidence that 17-(Allylamino)-17-demethoxygeldanamycin (17-AAG), an inhibitor of Hsp90 chaperone, decreases the rate of human adenovirus 5 (HAdV-5) replication in cell cultures by 95%. 17-AAG inhibited the transcription of early and late genes of HAdV-5, replication of viral DNA, and expression of viral proteins. 6 h after infection, Hsp90 inhibition results in a 6.3-fold reduction of the newly synthesized E1A protein level without a decrease in the E1A mRNA level. However, the Hsp90 inhibition does not increase the decay rate of the E1A protein that was constitutively expressed in the cell before exposure to the inhibitor. The co-immunoprecipitation proved that E1A protein interacted with Hsp90. Altogether, the presented results show, for the first time. that Hsp90 chaperones newly synthesized, but not mature, E1A protein. Because E1A serves as a transcriptional co-activator of adenovirus early genes, the anti-adenoviral activity of the Hsp90 inhibitor might be explained by the decreased E1A level. View Full-Text
Keywords: adenovirus; Hsp90; inhibitor; 17-AAG; E1A adenovirus; Hsp90; inhibitor; 17-AAG; E1A
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MDPI and ACS Style

Dalidowska, I.; Gazi, O.; Sulejczak, D.; Przybylski, M.; Bieganowski, P. Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus. Int. J. Mol. Sci. 2021, 22, 2020. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042020

AMA Style

Dalidowska I, Gazi O, Sulejczak D, Przybylski M, Bieganowski P. Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus. International Journal of Molecular Sciences. 2021; 22(4):2020. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042020

Chicago/Turabian Style

Dalidowska, Iga; Gazi, Olga; Sulejczak, Dorota; Przybylski, Maciej; Bieganowski, Pawel. 2021. "Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus" Int. J. Mol. Sci. 22, no. 4: 2020. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042020

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