Cytokinin-Controlled Gradient Distribution of Auxin in Arabidopsis Root Tip
Round 1
Reviewer 1 Report
The review is well written and organized. I do not feel that anything is missing or further needed. The only thing I would like to suggest is to remove the 'section' tags from the sub-headings.
Author Response
We gratefully appreciate for your valuable suggestion/ comment. We have deleted "section" tag in the revision.
Reviewer 2 Report
This is a well written and timely review of the cytokinin based regulatory system in the Arabidopsis root tip.
I am a little surprised that this review contains just the one figure and think that additional figure(s) providing an overall view of the root tip and the flow of phytohormones around the quiescent centre could be added. Perhaps the molecular cascades that constitute the regulatory mechanisms are also worthy of illustration to improve comprehension. A reference to Fig 1 needs to be added into the text, at least in section 2.
Minor corrections:
Line 9-11 A key component regulating this growth and development is the finely tuned cross-talk between the auxin and cytokinin phytohormones.
Line 12 but also varies root growth in response
Line 13 light on the molecular
Line 16 mechanisms underlying the cytokinin-controlled auxin gradient in the primary root.
Line 22 usually located below
Line 39 Delete “by intense investigation”.
Lines 42, 56, 124,152, 244 The section headings are a bit confusing. Eg 2. Cytokinin signal pathway, is all that is needed. Delete “Section 1:”
Line 43 . . in plants is similar to the bacterial . . .
Line 48 , which enters the nucleus
Line 57 genetic evidence
Line 80 Later, the authors found that . . .
Line 110 when the plant is treated . .
Line 125 In Arabidopsis, the GH3 . .
Line 127 IAA to IAA amino acid conjugates. The conjugation of amino acids with IAA is . . .
Line 128 one that can be . .
Line 139 If the LRC is lost, . . .
Line 150 . . amino acid conjugates, thus regulating the size of the root meristem.
Line 152/3 . .three protein families responsible for membrane trafficking:
Line 157 family proteins, involved in the
Line 159 directing
Line 169 in the cytokinin interaction
Line 172 depended
Line 179 polypeptide chain, the PINs family
Line 183 are expressed and active in the root tip.
Line 186 thereby modulating auxin distribution
Line 222 down-regulates PAT by
Line 232 LRC, where it plays a key role in maintaining
Line 235 genes act downstream
Line 239 CHIP assays showed
Line 245 In addition to the above mentioned PINs
Line 248 There are seven known members of the PILS family.
Line 259 leading to reduced transcriptional regulation
Line 278 , however, the details require further study.
Line 282 which is derived from
Line 284 the meristem of the root tip reaches a stable size,
Line 294 auxin concentration is maximal at the QC of
Line 299 root tip depended on
Line 308 controls the flow of auxin to the root tip
Line 312 In addition, the auxin reflux mechanism gradually decreases auxin when
Line 317 (MS) assay was applied to analyse
Line 318 each tissue of the root tip. Cytokinin was found to be mainly concentrated
Line 319/20 cells, and in the epidermis of the root tip, forming a gradient down to a maxima at the root cap.
Line 236 indicated
Line 237 from the above-ground parts
Line 333 In Section 2 we reviewed several
Author Response
We gratefully appreciate for your valuable suggestion/ comment. In the revision, we added Figure2 to provide an overall view of the root tip and the flow of phytohormones around the QC; added reference to Figure 1 and Figure 2 into the text. In addition, the mistakes in MS have been corrected one by one.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
