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Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates

1
Department of Oncological Endocrinology, Medical University of Łódź, 90-752 Łódź, Poland
2
Polish Mother’s Memorial Hospital—Research Institute, 93-338 Łódź, Poland
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2020, 17(18), 6841; https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph17186841
Received: 27 July 2020 / Revised: 8 September 2020 / Accepted: 15 September 2020 / Published: 18 September 2020
(This article belongs to the Special Issue Metal Exposure and Health Risk Assessment 2020)
The Fenton reaction (Fe2++H2O2→Fe3++OH+OH-) results in strong oxidative damage to macromolecules when iron (Fe) or hydrogen peroxide (H2O2) are in excess. This study aims at comparing Fe2++H2O2-induced oxidative damage to membrane lipids (lipid peroxidation, LPO) and protective effects of 17β-estradiol (a potential antioxidant) in porcine ovary and thyroid homogenates. Iron, as one of the Fenton reaction substrates, was used in the highest achievable concentrations. Thyroid or ovary homogenates were incubated in the presence of: (1st) FeSO4+H2O2 with/without 17β-estradiol (1 mM; 100, 10.0, 1.0 µM; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM); five experiments were performed with different FeSO4 concentrations (2400, 1200, 600, 300, 150 µM); (2nd) FeSO4 (2400, 1200, 600, 300, 150 µM)+H2O2 with/without 17β-estradiol; three experiments were performed with three highest 17β-estradiol concentrations; (3rd) FeSO4 (2400, 1200, 1100, 1000, 900, 800, 700, 600, 300, 150, 75 µM)+H2O2 (5 mM). LPO level [MDA+4-HDA/mg protein] was measured spectrophotometrically. The basal LPO level is lower in ovary than in thyroid homogenates. However, experimentally-induced LPO was higher in the former tissue, which was confirmed for the three highest Fe2+ concentrations (2400, 1200, 1100 µM). Exogenous 17β-estradiol (1 mM, 100, and 10 µM) reduced experimentally-induced LPO independently of iron concentration and that protective effect did not differ between tissues. The ovary, compared to the thyroid, reveals higher sensitivity to prooxidative effects of iron, however, it showed similar responsivity to protective 17β-estradiol activity. The therapeutic effect of 17β-estradiol against iron overload consequences should be considered with relation to both tissues. View Full-Text
Keywords: 17β-estradiol; Fenton reaction; ferrous; lipid peroxidation; oxidative damage 17β-estradiol; Fenton reaction; ferrous; lipid peroxidation; oxidative damage
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MDPI and ACS Style

Rynkowska, A.; Stępniak, J.; Karbownik-Lewińska, M. Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates. Int. J. Environ. Res. Public Health 2020, 17, 6841. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph17186841

AMA Style

Rynkowska A, Stępniak J, Karbownik-Lewińska M. Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates. International Journal of Environmental Research and Public Health. 2020; 17(18):6841. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph17186841

Chicago/Turabian Style

Rynkowska, Aleksandra, Jan Stępniak, and Małgorzata Karbownik-Lewińska. 2020. "Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates" International Journal of Environmental Research and Public Health 17, no. 18: 6841. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph17186841

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