Physiological and Biochemical Responses to Sublethal Concentrations of the Novel Pyropene Insecticide, Afidopyropen, in Whitefly Bemisia tabaci MED (Q Biotype)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Insecticides and Chemicals
2.3. Toxicities of the Eight Insecticides to B. tabaci
2.4. Sublethal Effects of Afidopyropen on B. tabaci
2.5. Metabolic Enzyme Assays
2.6. Statistical Analysis
3. Results
3.1. Toxicities of Eight Common Chemical Agents
3.2. Sublethal Effects of Afidopyropen on B. tabaci Biology
3.3. Activities of Metabolic Enzymes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Wang, X.W.; Li, P.; Liu, S.S. Whitefly interactions with plants. Curr. Opin. Insect Sci. 2017, 19, 70–75. [Google Scholar] [CrossRef]
- Horowitz, A.R.; Ghanim, M.; Roditakis, E.; Nauen, R.; Ishaaya, I. Insecticide resistance and its management in Bemisia tabaci species. J. Pest Sci. 2020, 93, 893–910. [Google Scholar] [CrossRef]
- Wei, J.; He, Y.Z.; Guo, Q.; Guo, T.; Liu, Y.Q.; Zhou, X.P.; Liu, S.S.; Wang, X.W. Vector development and vitellogenin determine the transovarial transmission of begomoviruses. Proc. Natl. Acad. Sci. USA. 2017, 114, 6746–6751. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Luo, C.; Yao, Y.; Wang, R.J.; Yan, F.M.; Hu, D.X.; Zhang, Z.L. The use of mitochondrial cytochrome oxidase mt COI gene sequences for the identification of biotypes of Bemisia tabaci (Gennadius) in China. Acta. Entomol. Sin. 2002, 45, 759–763. [Google Scholar]
- Chu, D.; Zhang, Y.J.; Brown, J.K.; Cong, B.; Xu, B.Y.; Wu, Q.J.; Zhu, G.R. The introduction of the exotic Q biotype of Bemisia tabaci from the Mediterranean region into China on ornamental crops. Fla. Entomol. 2006, 89, 168–174. [Google Scholar] [CrossRef]
- Pan, H.P.; Chu, D.; Ge, D.Q.; Wang, S.L.; Wu, Q.J.; Xie, W.; Jiao, X.G.; Liu, B.M.; Yang, X.; Yang, N.N.; et al. Further spread of and domination by Bemisia tabaci (Hemiptera: Aleyrodidae) biotype Q on field crops in China. J. Econ. Entomol. 2011, 104, 978–985. [Google Scholar] [CrossRef] [PubMed]
- Zheng, H.X.; Xie, W.; Wang, S.L.; Wu, Q.J.; Zhou, X.M.; Zhang, Y.J. Dynamic monitoring (B versus Q) and further resistance status of Q type Bemisia tabaci in China. Crop Prot. 2017, 94, 115–121. [Google Scholar] [CrossRef]
- Wang, S.L.; Zhang, Y.J.; Yang, X.; Xie, W.; Wu, Q.J. Resistance monitoring for eight insecticides on the sweet potato whitefly Bemisia tabaci in China. J. Econ. Entomol. 2017, 110, 660–666. [Google Scholar] [CrossRef]
- Wang, R.; Fang, Y.; Mu, C.Q.; Qu, C.; Li, F.Q.; Wang, Z.Y.; Luo, C. Baseline susceptibility and cross-resistance of cycloxaprid, a novel cis-nitromethylene neonicotinoid insecticide, in Bemisia tabaci MED from China. Crop Prot. 2018, 110, 283–287. [Google Scholar] [CrossRef]
- Wang, R.; Wang, J.D.; Che, W.N.; Sun, Y.; Li, W.X.; Luo, C. Characterization of field-evolved resistance to cyantraniliprole in Bemisia tabaci MED from China. J. Integr. Agr. 2019, 18, 2571–2578. [Google Scholar] [CrossRef]
- Kandasamy, R.; London, D.; Stam, L.; Von Deyn, W.; Zhao, X.; Salgado, V.L.; Nesterov, A. Afidopyropen: New and potent modulator of insect transient receptor potential channels. Insect Biochem. Mol. Biol. 2017, 84, 32–39. [Google Scholar] [CrossRef]
- Jeschke, P. Status and outlook for acaricide and insecticide discovery. Pest Manag. Sci. 2021, 77, 64–76. [Google Scholar] [CrossRef] [PubMed]
- Queiroz, O.D.S.; Nyoike, T.W.; Koch, R.L. Baseline susceptibility to afidopyropen of soybean aphid (Hemiptera: Aphididae) from the north central United States. Crop Prot. 2020, 129, 105020. [Google Scholar] [CrossRef]
- Zhang, Z.; Shi, H.J.; Xu, W.; Liu, J.T.; Geng, Z.Q.; Chu, D.; Guo, L. Pymetrozine-resistant whitefly Bemisia tabaci (Gennadius) populations in China remain susceptible to afidopyropen. Crop Prot. 2021, 149, 105757. [Google Scholar] [CrossRef]
- Slusher, E.K.; Cottrell, T.; Acebes-Doria, A.L. Effects of aphicides on pecan aphids and their parasitoids in pecan orchards. Insects 2021, 12, 241. [Google Scholar] [CrossRef] [PubMed]
- Chen, X.D.; Ashfaq, M.; Stelinski, L.L. Susceptibility of Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), to the insecticide afidopyropen: A new and potent modulator of insect transient receptor potential channels. Appl. Entomol. Zool. 2018, 53, 453–461. [Google Scholar] [CrossRef] [Green Version]
- Joseph, S.V. Repellent effects of insecticides on Stephanitis pyrioides Scott (Hemiptera: Tingidae) under laboratory conditions. Crop Prot. 2020, 127, 104985. [Google Scholar] [CrossRef]
- Koch, R.L.; Queiroz, O.D.S.; Aita, R.C.; Hodgson, E.W.; Potter, B.D.; Nyoike, T.; Ellers-Kirk, C.D. Efficacy of afidopyropen against soybean aphid (Hemiptera: Aphididae) and toxicity to natural enemies. Pest Manag. Sci. 2020, 76, 375–383. [Google Scholar] [CrossRef]
- Kumar, V.; McKenzie, C.L.; Osborne, L.S. Effect of foliar application of afidopyropen on Bemisia tabaci and Amblyseius swirskii. Arthropod Manage. Tests 2018, 43, tsy071. [Google Scholar]
- Ma, K.S.; Tang, Q.L.; Liang, P.Z.; Li, J.H.; Gao, X.W. Sublethal concentration of afidopyropen suppressed the population growth of the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae). J. Integr. Agr. 2021. Online. [Google Scholar] [CrossRef]
- Fang, Y.; Wang, J.D.; Luo, C.; Wang, R. Lethal and sublethal effects of clothianidin on the development and reproduction of Bemisia tabaci (Hemiptera: Aleyrodidae) MED and MEAM1. J. Insect Sci. 2018, 18, 37. [Google Scholar] [CrossRef] [Green Version]
- Wang, R.; Zhang, W.; Che, W.N.; Qu, C.; Li, F.Q.; Desneux, N.; Luo, C. Lethal and sublethal effects of cyantraniliprole, a new anthranilic diamide insecticide, on Bemisia tabaci (Hemiptera: Aleyrodidae) MED. Crop Prot. 2017, 91, 108–113. [Google Scholar] [CrossRef]
- Wang, R.; Zheng, H.X.; Qu, C.; Wang, Z.H.; Kong, Z.Q.; Luo, C. Lethal and sublethal effects of a novel cis-nitromethylene neonicotinoid insecticide, cycloxaprid, on Bemisia tabaci. Crop Prot. 2016, 83, 15–19. [Google Scholar] [CrossRef]
- Qu, C.; Zhang, W.; Li, F.Q.; Tetreau, G.; Luo, C.; Wang, R. Lethal and sublethal effects of dinotefuran on two invasive whiteflies, Bemisia tabaci (Hemiptera: Aleyrodidae). J. Asia Pac. Entomol. 2017, 20, 325–330. [Google Scholar] [CrossRef]
- Desneux, N.; Decourtye, A.; Delpuech, J.M. The sublethal effects of pesticides on beneficial arthropods. Annu. Rev. Entomol. 2007, 52, 81–106. [Google Scholar] [CrossRef] [PubMed]
- Wang, R.; Wang, J.D.; Zhang, J.S.; Che, W.N.; Luo, C. Characterization of flupyradifurone resistance in the whitefly Bemisia tabaci Mediterranean (Q biotype). Pest Manag. Sci. 2020, 76, 4286–4292. [Google Scholar] [CrossRef]
- LeOra Software. Polo Plus, A User’s Guide to Probit or Logit Analysis; LeOra Software: Berkeley, CA, USA, 2002. [Google Scholar]
- SPSS. Release 13.0 Version for Windows; SPSS: Chicago, IL, USA, 2011. [Google Scholar]
- Peng, Z.K.; Zheng, H.X.; Xie, W.; Wang, S.L.; Wu, Q.J.; Zhang, Y.J. Field resistance monitoring of the immature stages of the whitefly Bemisia tabaci to spirotetramat in China. Crop Prot. 2017, 98, 243–247. [Google Scholar] [CrossRef]
- Wang, R.; Wang, J.D.; Che, W.N.; Fang, Y.; Luo, C. Baseline susceptibility and biochemical mechanism of resistance to flupyradifurone in Bemisia tabaci. Crop Prot. 2020, 132, 105132. [Google Scholar] [CrossRef]
- Zhang, R.M.; Jang, E.B.; He, S.Y.; Chen, J.H. Lethal and sublethal effects of cyantraniliprole on Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Pest Manag. Sci. 2015, 71, 250–256. [Google Scholar] [CrossRef] [PubMed]
- Xiao, D.; Zhao, J.; Guo, X.J.; Li, S.; Wang, S. Sublethal effect of beta-cypermethrin on development and fertility of the Asian multicoloured ladybird beetle Harmonia axyridis. J. Appl. Entomol. 2016, 140, 598–608. [Google Scholar] [CrossRef]
- Sani, M.F.H.; Naseri, B.; Rafiee-Dastjerdi, H.; Aghajanzadeh, S.; Ghadamyari, M. Effects of three conventional insecticides on life table parameters and detoxifying enzymes activity of Pulvinaria aurantii Cockerell (Hemiptera: Coccidae). Toxin Rev. 2019. Online. [Google Scholar] [CrossRef]
- Li, W.Q.; Lu, Z.B.; Li, L.L.; Yu, Y.; Dong, S.; Men, X.Y.; Ye, B.H. Sublethal effects of imidacloprid on the performance of the bird cherry-oat aphid Rhopalosiphum padi. PLoS ONE 2018, 13, e0204097. [Google Scholar] [CrossRef]
- Lu, Y.H.; Zheng, X.S.; Gao, X.W. Sublethal effects of imidacloprid on the fecundity, longevity, and enzyme activity of Sitobion avenae (Fabricius) and Rhopalosiphum padi (Linnaeus). B. Entomol. Res. 2016, 106, 551–559. [Google Scholar] [CrossRef] [PubMed]
- Hernandez, E.P.; Kusakisako, K.; Talactac, M.R.; Galay, R.L.; Hatta, T.; Fujisaki, K.; Tsuji, N.; Tanaka, T. Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis. Parasit. Vectors 2018, 11, 460. [Google Scholar] [CrossRef] [PubMed]
- Zhang, N.; Liu, J.; Chen, S.N.; Huang, L.H.; Feng, Q.L.; Zheng, S.C. Expression profiles of glutathione S-transferase superfamily in Spodoptera litura tolerated to sublethal doses of chlorpyrifos. Insect Sci. 2016, 23, 675–687. [Google Scholar] [CrossRef]
- Lu, K.; Song, Y.Y.; Zeng, R.S. The role of cytochrome P450-mediated detoxification in insect adaptation to xenobiotics. Curr. Opin. Insect Sci. 2021, 43, 103–107. [Google Scholar] [CrossRef] [PubMed]
Insecticides | Number | Slope ± SE | LC50 (95% Fiducial Limits) (mg/L) | X2 (df) | p Values |
---|---|---|---|---|---|
Afidopyropen | 722 | 1.12 ± 0.10 | 7.38 (6.02–9.25) | 2.96 (4) | 0.59 |
Acetamiprid | 768 | 1.43 ± 0.15 | 9.85 (7.67–12.15) | 2.44 (4) | 0.73 |
Flonicamid | 740 | 1.19 ± 0.06 | 13.28 (1.72–2.42) | 2.05 (4) | 0.76 |
Flupyradifurone | 755 | 1.27 ± 0.08 | 18.74 (15.89–21.98) | 3.29 (4) | 0.55 |
Imidacloprid | 761 | 1.57 ± 0.11 | 26.37 (21.15–32.85) | 1.13 (4) | 0.88 |
Pymetrozine | 736 | 1.17 ± 0.07 | 116.37 (88.43–161.56) | 0.97 (4) | 0.91 |
Sulfoxaflor | 749 | 1.25 ± 0.09 | 11.73 (9.88–14.65) | 3.68 (4) | 0.47 |
Thiamethoxam | 767 | 1.09 ± 0.06 | 19.29 (16.39–24.42) | 3.29 (4) | 0.49 |
Strain | P450 Monooxygenase Activity | Esterase Activity | Glutathione S-Transferase Activity | |||
---|---|---|---|---|---|---|
pmol min−1 mg−1 Protein ± SE | Ratio b | nmol min−1 mg−1 Protein ± SE | Ratio b | nmol min−1 mg−1 Protein ± SE | Ratio b | |
CK | 8.94 ± 1.36 a | 26.45 ± 4.05 a | 786.33 ± 45.52 a | |||
LC10 | 10.15 ± 1.94 a | 1.14 | 27.92 ± 3.88 a | 1.06 | 822.93 ± 58.13 a | 1.05 |
LC25 | 10.67 ± 2.33 a | 1.19 | 25.84 ± 5.19 a | 0.98 | 1026.74 ± 51.88 b | 1.31 |
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Zhou, X.; Zhang, Z.; Zheng, H.; Zhang, Q.; Gong, J.; Li, C.; Wang, R. Physiological and Biochemical Responses to Sublethal Concentrations of the Novel Pyropene Insecticide, Afidopyropen, in Whitefly Bemisia tabaci MED (Q Biotype). Agronomy 2021, 11, 2260. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy11112260
Zhou X, Zhang Z, Zheng H, Zhang Q, Gong J, Li C, Wang R. Physiological and Biochemical Responses to Sublethal Concentrations of the Novel Pyropene Insecticide, Afidopyropen, in Whitefly Bemisia tabaci MED (Q Biotype). Agronomy. 2021; 11(11):2260. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy11112260
Chicago/Turabian StyleZhou, Xuan, Ziyi Zhang, Huixin Zheng, Qinghe Zhang, Jingyu Gong, Chuanren Li, and Ran Wang. 2021. "Physiological and Biochemical Responses to Sublethal Concentrations of the Novel Pyropene Insecticide, Afidopyropen, in Whitefly Bemisia tabaci MED (Q Biotype)" Agronomy 11, no. 11: 2260. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy11112260