Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Growth Conditions
2.2. Water Deficit Treatment
2.3. Photosynthetic Pigments and Chlorophyll Fluorescence Determination
2.4. Experimental Design and Data Analysis
3. Results
3.1. Chlorophyll and Carotenoids Contents
3.2. Fluorescence Parameters
3.3. Rapid Light-Response Curves
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Parameter | Abbreviation | Variation Source | |||||
---|---|---|---|---|---|---|---|
Vegetative Stage (55 DAE) | Reproductive Stage (65 DAE) | ||||||
Stage | Cultivar | Stage × Cultivar | Stage | Cultivar | Stage × Cultivar | ||
Total Chlorophyll | Chl total | *** | *** | *** | NS | *** | * |
Carotenoids | Cx + c | *** | *** | * | ** | *** | *** |
Maximum efficiency of PSII | Fv/Fm | *** | ** | ** | * | NS | NS |
Photochemical quenching | qP | NS | NS | NS | NS | * | NS |
Non-photochemical quenching | NPQ | NS | * | * | *** | *** | NS |
Initial slope | α | NS | ** | ** | NS | * | NS |
Maximum electron transport rate | ETRmax | NS | NS | NS | *** | *** | *** |
Light saturation point | Ik | NS | NS | ** | NS | * | NS |
Treatment | Vegetative Stage (55 DAE) | Treatment | Reproductive Stage (65 DAE) | ||||
---|---|---|---|---|---|---|---|
Fv/Fm | qP | NPQ | Fv/Fm | qP | NPQ | ||
Stress stage | Stress stage | ||||||
Control | 0.78 az | 0.61 | 1.10 | Control | 0.79 a | 0.60 | 1.07 |
Vegetative | 0.74 b | 0.60 | 1.23 | Reproductive | 0.75 b | 0.63 | 1.21 |
Significance | * | NS | NSy | NS | ** | NS | NS |
Cultivar | Cultivar | ||||||
Cerinza | 0.77 a | 0.63 | 1.12 | Cerinza | 0.77 | 0.56 | 1.27 ab |
Bachue | 0.76 ab | 0.63 | 0.89 | Bachue | 0.75 | 0.65 | 1.10 ab |
NUA35 | 0.74 b | 0.62 | 1.16 | NUA35 | 0.76 | 0.66 | 0.97 b |
Bacata | 0.77 ab | 0.57 | 1.58 | Bacata | 0.75 | 0.58 | 1.60 a |
Bianca | 0.77 ab | 0.58 | 1.29 | Bianca | 0.75 | 0.63 | 1.41 ab |
Significance | ** | NS | NS | Significance | NS | NS | *** |
Interaction | Interaction | ||||||
Cerinza × C | 0.79 a | 0.64 | 1.22 ab | Cerinza × C | 0.79 | 0.55 | 1.47 |
Bachue × C | 0.79 a | 0.66 | 0.60 b | Bachue × C | 0.80 | 0.65 | 0.78 |
NUA35 × C | 0.78 a | 0.62 | 1.07 ab | NUA35 × C | 0.79 | 0.71 | 0.50 |
Bacata × C | 0.79 a | 0.62 | 0.92 ab | Bacata × C | 0.78 | 0.54 | 1.25 |
Bianca × C | 0.76 a | 0.51 | 1.69 ab | Bianca × C | 0.79 | 0.57 | 1.35 |
Cerinza × V | 0.79 a | 0.58 | 1.26 ab | Cerinza × R | 0.77 | 0.57 | 0.98 |
Bachue × V | 0.74 ab | 0.64 | 1.23 ab | Bachue × R | 0.73 | 0.65 | 1.14 |
NUA35 × V | 0.67 b | 0.65 | 0.71 ab | NUA35 × R | 0.76 | 0.64 | 1.08 |
Bacata × V | 0.75 a | 0.54 | 1.86 a | Bacata × R | 0.73 | 0.66 | 1.56 |
Bianca × V | 0.77 a | 0.61 | 1.11 ab | Bianca × R | 0.77 | 0.62 | 1.27 |
Significance | * | NS | * | Significance | NS | NS | NS |
xCV (%) | 4.31 | 11.40 | 42.44 | CV (%) | 5.63 | 13.04 | 32.0 |
Vegetative Stage (55 DAE) | Reproductive Stage (65 DAE) | ||||||
---|---|---|---|---|---|---|---|
Treatment | α | ETRmax | Ik | Treatment | α | ETRmax | Ik |
(μmol∙m−2∙s−1) | (μmol∙m−2∙s−1) | (μmol∙m−2∙s−1) | (μmol∙m−2∙s−1) | ||||
Stress stage | Stress stage | ||||||
Control | 0.23 a z | 207.42 | 936.47 | Control | 0.24 | 190.38 a | 795.48 |
Vegetative | 0.21 ab | 191.87 | 876.60 | Reproductive | 0.22 | 165.71 b | 811.98 |
Significance | NSy | NS | NS | Significance | NS | *** | NS |
Interaction | Interaction | ||||||
Cerinza | 0.22 ab | 241.35 | 1122.83 | Cerinza | 0.22 b | 151.75 b | 768.83 ab |
Bachue | 0.24 a | 209.75 | 896.54 | Bachue | 0.23 ab | 194.09 a | 830.91 ab |
NUA35 | 0.22 ab | 204.39 | 916.10 | NUA35 | 0.24 a | 204.87 a | 893.33 ab |
Bacata | 0.19 b | 169.94 | 850.35 | Bacata | 0.19 b | 150.75 b | 685.04 b |
Bianca | 0.21 b | 203.09 | 976.53 | Bianca | 0.21 b | 188.75 a | 850.14 a |
Significance | ** | NS | NS | Significance | * | *** | * |
Interaction | Interaction | ||||||
Cerinza × C | 0.23 abc | 232.35 | 1052.72 ab | Cerinza × C | 0.21 | 167.64 bcd | 773.82 |
Bachue × C | 0.27 a | 221.03 | 838.06 ab | Bachue × C | 0.27 | 195.83 b | 728.18 |
NUA35 × C | 0.24 a | 185.92 | 786.85 b | NUA35 × C | 0.28 | 251.22 a | 910.54 |
Bacata × C | 0.23 abc | 207.85 | 884.87 ab | Bacata × C | 0.21 | 159.22 cd | 707.97 |
Bianca × C | 0.16 c | 189.95 | 1119.85 a | Bianca × C | 0.21 | 188.56 bc | 856.91 |
Cerinza × V | 0.21 abc | 201.21 | 953.87 ab | Cerinza × R | 0.23 | 145.86 d | 758.85 |
Bachue × V | 0.23 ab | 196.73 | 844.59 ab | Bachue × R | 0.20 | 196.91 bc | 905.40 |
NUA35 × V | 0.21 abc | 235.22 | 976.33 ab | NUA35 × R | 0.24 | 158.5 bcd | 774.97 |
Bacata × V | 0.17 bc | 148.54 | 838.19 ab | Bacata × R | 0.21 | 152.89 bcd | 718.17 |
Bianca × V | 0.23 abc | 177.65 | 769.99 b | Bianca × R | 0.21 | 188.93 bc | 902.48 |
Significance | ** | NS | ** | Significance | NS | *** | NS |
CVx (%) | 12.50 | 19.66 | 13.16 | CVx (%) | 14.22 | 10.21 | 13.76 |
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Sánchez-Reinoso, A.D.; Ligarreto-Moreno, G.A.; Restrepo-Díaz, H. Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean. Agronomy 2019, 9, 526. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy9090526
Sánchez-Reinoso AD, Ligarreto-Moreno GA, Restrepo-Díaz H. Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean. Agronomy. 2019; 9(9):526. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy9090526
Chicago/Turabian StyleSánchez-Reinoso, Alefsi David, Gustavo Adolfo Ligarreto-Moreno, and Hermann Restrepo-Díaz. 2019. "Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean" Agronomy 9, no. 9: 526. https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy9090526