New Transcriptome-Based SNP Markers for Noug (Guizotia abyssinica) and Their Conversion to KASP Markers for Population Genetics Analyses
, Rodomiro Ortiz 1, Eva Johansson 1, Endashaw Bekele 2, Kassahun Tesfaye 2,3, Cecilia Hammenhag 1 and Mulatu Geleta 1
Round 1
Reviewer 1 Report
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Comments for author File: 
Comments.pdf
Author Response
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Author Response File: Author Response.pdf
Reviewer 2 Report
Overall study is well designed and well written. Authors can provide information about the SNPs that were identified in two of the lines that were subjected to transcriptome sequencing. Also it would be good to discuss why the success rate of KASP marker was low? This could be due to the heterozygous nature of the SNPs identified and also possibly coverage an issue for allele. Also why the number of SNPs is so low among these accessions or lines.
Author Response
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Author Response File: Author Response.pdf
Reviewer 3 Report
This research from Tsehay et al highlight important findings with potential applications for future crop improvement efforts in Noug crop. The authors have done a good job presenting the background and the rationale for the study. The manuscript is scientifically sound and clearly presented. However, I would like to highlight a few minor points here to get authors response. Particularly, I would like to see a comment on validation efforts required to assess the validity of the developed KASP markers. It would be interesting to see the performance of these markers on a set of new lines.
Some additional comments can be found in the attached pdf document.
Comments for author File: Comments.pdf
Author Response
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Author Response File: Author Response.pdf
