Medium Optimization for GA4 Production by Gibberella fujikuroi Using Response Surface Methodology
Round 1
Reviewer 1 Report
Include a list of all acronyms used in the manuscript, and all acronyms should be defined at the point of first use.
Page 1, line 13: italicize Gibberella fujikuroi.
Page 1, line 15: change method to methodology.
Page 1, line 18: The results indicated ...
Page 1, line 41: write G. fujikuroi in full at the first point of usage.
Page 1, line 42: Change "One aims to improve..." to "One way to improve...".
For consistency, GA4 should be written as GA4 all through the manuscript.
Page 2, line 46: It was shown that the DES knockout...
Page 2, line 46: Start a new paragraph with "Optimization of fermentation conditions....
Page 2, line 67: bacteria???
Page 2, line 81: Methanol used was....
Page 2, line 91:.. at 220 rpm and 33 oC for...
Page 3, line 104: Check the statement here. Nine not ten oils were listed here.
Page 3, line 106: ...for further optimization studies.
Page 3, lines 111-112: The experimental factors and their levels are listed in Table 1.
Table 1: put the % in parentheses in the first column, e.g., A(%), B(%) and C(%).
Page 4, line 135: All tables should be numbered sequentially. Table 9 should be renumbered as Table 3.
Page 4, line 150: The number of oils listed are 9 and not 10.
Figure 1 makes no sense. First, there are 11 oil samples displayed. Second, there is no key to indicate the oil type.
The titles of tables 3, 6, and 9 should be corrected. The actual values of the variables were not provided. My suggestion is that the actual values should be provided instead of the coded values. For instance, the title should then read "Table 3. Experimental design of three independent variables and their and actual values of GA4 production using BBD. "
Page 6, lines 183-185 should come before line 176.
All equations must be numbered.
Page 6, lines 178: What is incoherence? Do you mean coefficient of determination (R2)?
Table4: There are too many insignificant terms, any explanation?
Tables 5 and 11 should be deleted. These are single row tables.
Page 8, line 247: R2 should be R2. ...and the R2 value was 0.962, which indicates that the fitted model is significant...
Note under Table 7: R2 should be R2.
Page 12, line 347: This may be as a result of the metabolic flux distribution change ...
The conclusion needs to be rewritten. The statements below are not part of the current study.
"Subsequently, GA4 biosynthesis and global regulation analyses were conducted using transcriptome, proteome, and metabolomics. Thus, the product synthesis mechanism can be explored to identify novel targets for increasing GA4 production."
Author Response
Dear Editor:
Thank you for your E-mail in which you send us the reviewers’ comments on our manuscript fermentation-1704385 entitled "Medium Optimization for GA4 Production by Gibberella fujikuroi using Response Surface Methodology". We have extensively revised the manuscript according to the reviewers’ comments. Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The aim of the study has been clearly presented and the background of this issue comprehensively described. It seems quite relevant to scientific community to investigate the factors influencing gibberellin synthesis including A4 molecule via microbial methods. The strong advantage of the paper is the consequence in appropriate use of DoE methods applied in conducted experiments.
I would reccommend some improvements in the manuscript:
- Please add some details on Giberella fujikuroi. Isn't it Fusarium? Not many microbes can utilize lipids, so this is a crucial ability for the species and can be also underlined in the introduction.
- Please remove text lines 145 - 147.
- It is not clear why did you tried to use a mix of three oils and than you compared two of them? Why do not use oils individually in the culture medium? Do those oils contain some ingredients which are necessary in GA4 biosynthesis?
- Please change commas into dots e.g. table 4, 10
- Describe what letters A, B, C, A2 etc. mean in equation (lines 185, 273). Do they not need to be numbered?
- What does asteriscs mean in Table 10?
- I am not sure why you mention eleven results in Table 11? There is only three scores - 1, 2 and 3?
- Please write species names italics in reference articles' titles.
Author Response
Dear Editor:
Thank you for your E-mail in which you send us the reviewers’ comments on our manuscript fermentation-1704385 entitled "Medium Optimization for GA4 Production by Gibberella fujikuroi using Response Surface Methodology". We have extensively revised the manuscript according to the reviewers’ comments. Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
The authors should check the attached annotated file for some minor corrections.
Comments for author File: Comments.pdf
Reviewer 2 Report
Dear Authors I would like to thank you for all explanations. The manuscript has been carefully revised.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
