Next Article in Journal
Ultrasonographic Findings in Common Thyroid and Parathyroid Disorders—Advantages of Real Time Observation by the Endocrinologist with their Own Ultrasound Machine
Previous Article in Journal
Metagenome Techniques for Detection of Pathogens Causing Ocular Infection
Brief Report

AHR:IKAROS Interaction Promotes Platelet Biogenesis in Response to SR1

1
Institut National de la Santé et de la Recherche Médicale (INSERM), Etablissement Français du Sang (EFS)-Grand Est, Biologie et Pharmacologie des Plaquettes Sanguines (BPPS) Unité Mixte de Recherche (UMR)_S1225, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, F-67000 Strasbourg, France
2
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR) 7104, Université de Strasbourg, F-67404 Illkirch, France
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this study and were considered as co-first authors.
Academic Editor: Toshio Hattori
Received: 15 December 2020 / Revised: 10 February 2021 / Accepted: 18 February 2021 / Published: 2 March 2021
In vitro, the differentiation of megakaryocytes (MKs) is improved by aryl-hydrocarbon receptor (AHR) antagonists such as StemRegenin 1 (SR1), an effect physiologically recapitulated by the presence of stromal mesenchymal cells (MSC). This inhibition promotes the amplification of a CD34+CD41low population able to mature as MKs with a high capacity for platelet production. In this short report, we showed that the emergence of the thrombocytogenic precursors and the enhancement of platelet production triggered by SR1 involved IKAROS. The downregulation/inhibition of IKAROS (shRNA or lenalidomide) significantly reduced the emergence of SR1-induced thrombocytogenic population, suggesting a crosstalk between AHR and IKAROS. Interestingly, using a proximity ligation assay, we could demonstrate a physical interaction between AHR and IKAROS. This interaction was also observed in the megakaryocytic cells differentiated in the presence of MSCs. In conclusion, our study revealed a previously unknown AHR/ IKAROS -dependent pathway which prompted the expansion of the thrombocytogenic precursors. This AHR- IKAROS dependent checkpoint controlling MK maturation opens new perspectives to platelet production engineering. View Full-Text
Keywords: AHR; IKAROS; megakaryocytes; platelet production AHR; IKAROS; megakaryocytes; platelet production
Show Figures

Figure 1

MDPI and ACS Style

Mallo, L.; Do Sacramento, V.; Gachet, C.; Chan, S.; Kastner, P.; Lanza, F.; de la Salle, H.; Strassel, C. AHR:IKAROS Interaction Promotes Platelet Biogenesis in Response to SR1. Reports 2021, 4, 7. https://0-doi-org.brum.beds.ac.uk/10.3390/reports4010007

AMA Style

Mallo L, Do Sacramento V, Gachet C, Chan S, Kastner P, Lanza F, de la Salle H, Strassel C. AHR:IKAROS Interaction Promotes Platelet Biogenesis in Response to SR1. Reports. 2021; 4(1):7. https://0-doi-org.brum.beds.ac.uk/10.3390/reports4010007

Chicago/Turabian Style

Mallo, Lea, Valentin Do Sacramento, Christian Gachet, Susan Chan, Philippe Kastner, François Lanza, Henri de la Salle, and Catherine Strassel. 2021. "AHR:IKAROS Interaction Promotes Platelet Biogenesis in Response to SR1" Reports 4, no. 1: 7. https://0-doi-org.brum.beds.ac.uk/10.3390/reports4010007

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop