Meat Quality and Protein Expression in Livestock and Poultry

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Nutrition".

Deadline for manuscript submissions: closed (15 June 2022) | Viewed by 22189

Special Issue Editor


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Guest Editor
Department of Animal Genetics, Breeding and Ethology, Faculty of Animal Science, University of Agriculture in Krakow, Krakow, Poland
Interests: meat quality; muscle structure; ovary; ovarian follicles; cell biology; histology; histopathology; immunohistochemistry; protein expression; apoptosis; oxidative stress
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Special Issue Information

Dear Colleagues,

Muscle is a major source of protein for humans. Currently, as food supplies are more plentiful, meat quality is becoming increasingly important to consumers. Today, we know that meat quality is the result of many factors, such as animal nutrition, rearing and slaughter, and genotype. The long-term intensive selection of livestock and poultry, aimed at improving weight gain and dressing percentage in increasingly short periods of time, has contributed to undesirable metabolic and physiological disorders in the muscle tissue microstructure, which have adversely affected meat quality. Theories concerning the problem of increasing meatiness and decreasing meat quality in livestock and poultry often use research on muscle tissue microstructures and genetics at the molecular level. It is exactly these muscle microstructure parameters, in particular, muscle fiber composition, intramuscular fat content, and intramuscular connective tissue, that determine the physico-chemical parameters of meat (such as acidity, color, tenderness, drip loss, and water holding capacity) and thus its quality. It is generally accepted that during the conversion of muscle to meat, postmortem changes that occur in the muscle tissue and determine the subsequent production and culinary usefulness of the meat are dependent, among other things, on the rate of myofibrillar and cytoskeletal protein degradation. In the last decade, proteomic analyses have shown that protein degradation is associated with energy metabolism, cellular death cascades (apoptosis or autophagy), cellular survival (heat shock and chaperone proteins), and oxidative stress. Elucidation of these processes and their impacts on postmortem muscle proteins, as well as their inherent interactions, is key to understanding the mechanisms that influence meat quality.

This Special Issue welcomes both review and research papers on all aspects of the relationship between meat structure, protein expression, and meat quality. Areas of interest include the effect of breed, line, gender, nutrition, aging time, muscle type, etc. on meat quality and its structure and rate of protein degradation. Topics of special interest are the muscle development and growth mechanisms of livestock and poultry as related to muscle myopathies and meat quality.

I invite you to share your recent findings in this Special Issue.

Prof. Dorota Wojtysiak
Guest Editor

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Keywords

  • meat quality
  • microstructure
  • muscle type
  • skeletal muscle proteins
  • postmortem aging
  • muscle myopathies
  • apoptosis
  • oxidative stress
  • heat shock proteins
  • breed
  • nutrition

Published Papers (9 papers)

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Research

11 pages, 3099 KiB  
Article
Transcriptome Analysis of the Adipose Tissue of Luchuan and Duroc Pigs
by Hongyuan Pan, Tengda Huang, Lin Yu, Peng Wang, Songtao Su, Tian Wu, Yin Bai, Yonghao Teng, Yutian Wei, Lei Zhou and Yixing Li
Animals 2022, 12(17), 2258; https://0-doi-org.brum.beds.ac.uk/10.3390/ani12172258 - 31 Aug 2022
Cited by 1 | Viewed by 1662
Abstract
Fat deposition is a crucial element in pig production that affects production efficiency, quality and consumer choices. In this study, Duroc pigs, a Western, famous lean pig breed, and Luchuan pigs, a Chinese, native obese pig breed, were used as animal materials. Transcriptome [...] Read more.
Fat deposition is a crucial element in pig production that affects production efficiency, quality and consumer choices. In this study, Duroc pigs, a Western, famous lean pig breed, and Luchuan pigs, a Chinese, native obese pig breed, were used as animal materials. Transcriptome sequencing was used to compare the back adipose tissue of Duroc and Luchuan pigs, to explore the key genes regulating fat deposition. The results showed that 418 genes were highly expressed in the Duroc pig, and 441 genes were highly expressed in the Luchuan pig. In addition, the function enrichment analysis disclosed that the DEGs had been primarily enriched in lipid metabolism, storage and transport pathways. Furthermore, significant differences in the metabolic pathways of alpha-linolenic acid, linoleic acid and arachidonic acid explained the differences in the flavor of the two kinds of pork. Finally, the gene set enrichment analysis (GSEA) exposed that the difference in fat deposition between Duroc and Luchuan pigs may be due to the differential regulation of the metabolism pathway of fatty acid. Therefore, this study described the differential expression transcriptional map of adipose tissue of Duroc pig and Luchuan pig, identified the functional genes regulating pig fat deposition, and provided new hypotheses and references for further study of fat development. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
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11 pages, 438 KiB  
Article
The g.4290 C>G Polymorphism in the FADS2 Gene Modifies the Fatty Acid Profile of the Pectoralis Superficialis Muscle of Ross 308 Broiler Chickens
by Urszula Kaczor, Sebastian Sawicki, Joanna Nowak, Julia Gabryś, Jakub Jurczyk, Dorota Wojtysiak and Katarzyna Połtowicz
Animals 2022, 12(15), 1882; https://0-doi-org.brum.beds.ac.uk/10.3390/ani12151882 - 22 Jul 2022
Viewed by 1210
Abstract
The effect of the g.4290 C>G substitution in the FADS2 gene and g.285 C>T in the FABP4 gene on carcass quality, meat quality, and fatty acid profile of the pectoralis superficialis muscle of 238 male broiler chickens reared up to 45 days of [...] Read more.
The effect of the g.4290 C>G substitution in the FADS2 gene and g.285 C>T in the FABP4 gene on carcass quality, meat quality, and fatty acid profile of the pectoralis superficialis muscle of 238 male broiler chickens reared up to 45 days of age was analyzed. A significant influence of g.4290 C>G in the FADS2 gene on the pectoralis superficialis muscle fatty acid profile was demonstrated. Chickens with the GG genotype were characterized by the highest content of conjugated linoleic acid, amino acids, eicosapentaenoic acids, docosapentaenoic acid, docosahexaenoic acids. and the lowest value of the linoleic acid/alpha-linolenic acid ratio. The FABP4 polymorphism determined only the content of C18:1n-9, C18:2n-6 and docosahexaenoic acid. There was no effect of the studied genotypes on final body weight, carcass quality traits, or quality of broiler pectoral muscles. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
8 pages, 1686 KiB  
Article
Association of Serum Glucose, Serotonin, Aspartate Aminotransferase, and Calcium Levels with Meat Quality and Palatability Characteristics of Broiler Pectoralis Major Muscle
by Boin Lee and Young Min Choi
Animals 2022, 12(12), 1567; https://0-doi-org.brum.beds.ac.uk/10.3390/ani12121567 - 17 Jun 2022
Viewed by 1439
Abstract
This study investigated the correlations between apoptosis-related blood biochemical parameters measured at exsanguination and the meat and sensory quality characteristics of broiler pectoralis major muscle. The concentration of serotonin showed a positive correlation with aspartate aminotransferase (AST) activity (p < 0.001) and [...] Read more.
This study investigated the correlations between apoptosis-related blood biochemical parameters measured at exsanguination and the meat and sensory quality characteristics of broiler pectoralis major muscle. The concentration of serotonin showed a positive correlation with aspartate aminotransferase (AST) activity (p < 0.001) and a negative correlation with calcium content (p < 0.01). All serum parameters showed relationships with muscle pH at 15 min and/or 24 h postmortem (p < 0.05). Serum AST activity, which had a negative correlation with calcium content (p < 0.01), was positively related with muscle pH and negatively correlated with Warner–Bratzler shear force values (WBS, p < 0.05). Principal component analysis results revealed the associations between AST activity and meat quality traits, including pH24h, lightness, and WBS. Furthermore, cooked breast with higher AST activity and lower calcium level tended to exhibit higher scores of tenderness and overall acceptability than that with lower AST activity and higher calcium level (p < 0.05). Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
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13 pages, 330 KiB  
Article
Effect of Concentration of Flaxseed (Linum usitatissimum) and Duration of Administration on Fatty Acid Profile, and Oxidative Stability of Pork Meat
by Martin Bartkovský, Drahomíra Sopková, Zuzana Andrejčáková, Radoslava Vlčková, Boris Semjon, Slavomír Marcinčák, Lukáš Bujňák, Matej Pospiech, Jozef Nagy, Peter Popelka and Petronela Kyzeková
Animals 2022, 12(9), 1087; https://0-doi-org.brum.beds.ac.uk/10.3390/ani12091087 - 22 Apr 2022
Cited by 5 | Viewed by 1735
Abstract
Flaxseed is a common ingredient used for livestock feed. The aim of this work was to study the effect of a diet supplemented with flaxseed at 5% and 10% concentrations in the intervals of 3 and 6 weeks prior slaughter on fatty acid [...] Read more.
Flaxseed is a common ingredient used for livestock feed. The aim of this work was to study the effect of a diet supplemented with flaxseed at 5% and 10% concentrations in the intervals of 3 and 6 weeks prior slaughter on fatty acid profile and oxidative stability of pork meat. Meat samples were collected after slaughter from each animal (five groups, n = 6). Samples of the musculus longissimus dorsi (MLD) and the musculus gluteobiceps (MGB) were selected. Chemical composition, fatty acid profile and oxidative stability during the storage of meat under chilling conditions (4 °C, 7 days) was analyzed. The addition of flaxseed significantly affected the composition of fatty acid profile and the shelf life of the produced meat. The fat content was changed in the experimental groups with 10% flaxseed supplementation (10.84% in MGB and 9.56% MLD) versus the control group. Despite the different concentrations of flaxseed, the best EPA/AA ratio was observed in the experimental groups fed with flaxseed supplementation for 3 weeks. The worst oxidative stability of meat samples (p < 0.05) was recorded in the experimental groups with the addition of flaxseed for 6 weeks, which was related to higher PUFA content in samples of the experimental groups and higher susceptibility of PUFAs to lipid oxidation. The oxidative stability of meat in the experimental group fed 5% flaxseed supplementation for 3 weeks was not affected. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
12 pages, 303 KiB  
Article
Effect of a Diet Supplemented with Nettle (Urtica dioica L.) or Fenugreek (Trigonella Foenum-Graecum L.) on the Content of Selected Heavy Metals in Liver and Rabbit Meat
by Sylwia Ewa Pałka, Ewa Drąg-Kozak, Łukasz Migdał and Michał Kmiecik
Animals 2022, 12(7), 827; https://0-doi-org.brum.beds.ac.uk/10.3390/ani12070827 - 24 Mar 2022
Cited by 3 | Viewed by 2324
Abstract
The literature on herbal additives for rabbit feed offers little information on the use of nettle and fenugreek. Both of these herbs are valuable sources of vitamins and minerals. These herbs affect the growth, health, and meat quality of rabbits. They regulate the [...] Read more.
The literature on herbal additives for rabbit feed offers little information on the use of nettle and fenugreek. Both of these herbs are valuable sources of vitamins and minerals. These herbs affect the growth, health, and meat quality of rabbits. They regulate the digestive system, stimulate the appetite, have a positive effect on the functioning of the immune system, and exhibit antibacterial activity. The purpose of the present study was to determine the effect of nettle (Urtica dioica L.) leaves or fenugreek (Trigonella foenum-graecum L.) seeds in the feed on the content of selected heavy metals in the liver and meat of the rabbit. The rabbits were divided into three groups: group C (n = 20; 10♂ and 10♀) was fed ad libitum with a complete feed, N group (n = 20; 10♂ and 10♀) was fed a complete mixture with 1% added nettle, and group F (n = 20; 10♂ and 10♀) was fed with a complete mixture with 1% added fenugreek. The experiment lasted 7 weeks (from the 35th to the 84th day of the rabbits’ lives). All the rabbits were slaughtered on the 84th day of age, with a body weight of about 2.6 kg. The concentration of heavy metals (Zn, Cu, Ni, Mn, Fe, Pb, Cd) was determined by the atomic absorption spectrometry (AAS). The additives to the feed significantly affected the content of elements in both the liver and the meat of rabbits (p < 0.05). The highest level of the heavy metals, regardless of the used diet, was recorded in the liver (p < 0.05). The meat (m. longissimus lumborum) and the liver of rabbits fed with herbal fodder contained less tested metals than in animals fed with fodder without additives (p < 0.05). Moreover, more essential metals were found in the liver of rabbits fed with fenugreek than rabbits fed with nettle. In the meat and liver of rabbits, the permissible content of cadmium and lead was not exceeded. Additionally, male livers had a significantly higher content of copper and manganese compared to female livers (p < 0.05). It is important to study the content of heavy metals in the used animal herbal feed additives and their interaction with each other, as they affect the distribution of elements in tissues and organs. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
12 pages, 1558 KiB  
Article
Associations of Apoptotic and Anti-Apoptotic Factors with Beef Quality, Histochemical Characteristics, and Palatability of Hanwoo Longissimus thoracis Muscle
by Boin Lee, Jae-Yeong Kim and Young-Min Choi
Animals 2022, 12(4), 467; https://0-doi-org.brum.beds.ac.uk/10.3390/ani12040467 - 14 Feb 2022
Cited by 4 | Viewed by 1321
Abstract
This study compared the meat quality, histochemical traits, palatability, and expression levels of apoptotic (cytochrome c and caspases) and anti-apoptotic (small heat shock proteins) factors at 45 min and 24 h post-mortem of Hanwoo Longissimus thoracis muscles in groups categorized by Warner-Bratzler [...] Read more.
This study compared the meat quality, histochemical traits, palatability, and expression levels of apoptotic (cytochrome c and caspases) and anti-apoptotic (small heat shock proteins) factors at 45 min and 24 h post-mortem of Hanwoo Longissimus thoracis muscles in groups categorized by Warner-Bratzler shear force (WBS) values to investigate the association between beef tenderness variation and apoptosis-related molecules. There were no differences in marbling scores, meat quality traits, or histochemical characteristics among the WBS groups (p > 0.05) indicating no significant effect on the tenderness variation in the current study. On the other hand, the low group exhibited higher levels of apoptotic and anti-apoptotic factors (except for αβ-crystallin) at 45 min post-mortem compared to the high WBS group, resulting in higher scores of tenderness attributes (p < 0.05). However, the level of αβ-crystallin at 45 min post-mortem was lower in the low and medium WBS groups compared to the high WBS group (p < 0.0106). At 24 h post-mortem, no significant differences were observed in the expression levels of apoptosis-related factors among the WBS groups (p > 0.05) except for heat shock protein 27 (p < 0.05). Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
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12 pages, 636 KiB  
Article
Growth Performance, Carcass Characteristics and Meat Quality of Organically Reared Broiler Chickens Depending on Sex
by Dorota Cygan-Szczegielniak and Joanna Bogucka
Animals 2021, 11(11), 3274; https://0-doi-org.brum.beds.ac.uk/10.3390/ani11113274 - 16 Nov 2021
Cited by 15 | Viewed by 2721
Abstract
Given the growing interest of consumers in naturally produced meat, we decided to examine the muscles of Ross 308 broiler chickens kept in an ecological system, with the division into two research groups depending on sex. All the analyses were carried out using [...] Read more.
Given the growing interest of consumers in naturally produced meat, we decided to examine the muscles of Ross 308 broiler chickens kept in an ecological system, with the division into two research groups depending on sex. All the analyses were carried out using the appropriate methods recommended by the AOAC and in accordance with the Polish standards (PN), which are described in detail in the relevant section of the publication. The aim of the experiment was to investigate the effect of sex on the growth performance, carcass traits and meat quality, as well as the fatty acid profile and histological parameters, of the pectoral muscles from organic broiler chickens. A total of 60 one-day-old Ross 308 chickens (half males and half females) were divided into two groups, according to the sex, and reared under organic conditions (Org.) until 82 days of age (ten birds, i.e., five males and five females in each of three pens; replications for experimental groups). Compared with the female group, the male group had a higher final BW and carcass weight (p < 0.05). The males had a better growth and slaughter performance than the females. The meat quality traits and fatty acids content were also affected by sex. The meat from females showed a significantly higher (p < 0.05) protein, dry matter, fiber diameter and shear force and a significantly lower (p < 0.05) fat level than the male group. In this research, the suitability of these fast-growing broilers for natural and organic production systems has been researched with regard to the performance, meat quality and histological characteristics of the muscles. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
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11 pages, 1118 KiB  
Article
Effects of Probiotics, Prebiotics and Synbiotics Injected in Ovo on the Microstructure of the Breast Muscle in Different Chicken Genotypes
by Karolina Stasiak, Anna Slawinska and Joanna Bogucka
Animals 2021, 11(10), 2944; https://0-doi-org.brum.beds.ac.uk/10.3390/ani11102944 - 12 Oct 2021
Cited by 4 | Viewed by 1993
Abstract
The aim of the study was to analyse the effect of probiotics, prebiotics and synbiotics injected in ovo on day 12 of embryonic development on the microstructure of the superficial pectoral muscle (musculus pectoralis superficialis) from 42-day-old chickens of different genotypes: broilers (Ross [...] Read more.
The aim of the study was to analyse the effect of probiotics, prebiotics and synbiotics injected in ovo on day 12 of embryonic development on the microstructure of the superficial pectoral muscle (musculus pectoralis superficialis) from 42-day-old chickens of different genotypes: broilers (Ross 308) and general-purpose type (green-legged partridge (GP) chickens Zk-11, native chickens). Incubated eggs were divided into four groups (each genotype separately) depending on the substance injected in ovo: normal saline (C, control); Lactococcus lactis subsp. cremoris (PRO); galactooligosaccharides, GOS (PRE) or GOS + L. lactis (SYN). After hatching, chicks were placed in eight replicated pens (four pens/genotype group). There were eight birds per pen. In total, 64 birds were used in the experiment. Birds were slaughtered at the age of 42 days, and samples of superficial pectoral muscles were taken for analysis. The microstructure of the pectoral muscles was evaluated using the cryosectioning (frozen tissue sectioning) technique and staining with haematoxylin and eosin. Statistical analysis revealed that the in ovo injection of probiotics, prebiotics and synbiotics had no significant effect on the diameter of muscle fibres from chickens of the two genotypes. The number of fibres in the muscles from green-legged partridge chickens was about three-fold higher than the fibre density in the muscles from broiler chickens, with the fibre diameter being two-fold smaller. This fact may indicate a greater tenderness of meat from GP chickens compared to the meat from Ross 308 broilers. In the case of broilers, a prebiotic (GOS) was the most effective bioactive substance in reducing the number of histopathological changes. Considering muscles from GP chickens, the number of normal fibres was highest in birds treated with the probiotic. These findings indicate that the microstructural features of pectoral muscles depend not only on the type of the injected bioactive substance but also on the genotype of chickens. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
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14 pages, 269 KiB  
Article
Effect of a Diet Supplemented with Nettle (Urtica dioica L.) or Fenugreek (Trigonella foenum-graecum L.) on the Post-Slaughter Traits and Meat Quality Parameters of Termond White Rabbits
by Sylwia Ewa Pałka, Agnieszka Otwinowska-Mindur, Łukasz Migdał, Michał Kmiecik and Dorota Wojtysiak
Animals 2021, 11(6), 1566; https://0-doi-org.brum.beds.ac.uk/10.3390/ani11061566 - 27 May 2021
Cited by 10 | Viewed by 5521
Abstract
The purpose of the study was to analyse the effect of nettle (Urtica dioica L.) leaves and fenugreek (Trigonella foenum-graecum L.) seeds as additives to fodder in order to improve post-slaughter traits and quality parameters of Termond White rabbit meat (n [...] Read more.
The purpose of the study was to analyse the effect of nettle (Urtica dioica L.) leaves and fenugreek (Trigonella foenum-graecum L.) seeds as additives to fodder in order to improve post-slaughter traits and quality parameters of Termond White rabbit meat (n = 60; 30♂, 30♀). Three experimental groups were created. The control group (n = 20; 10♂ and 10♀) was fed ad libitum feed containing corn, bran, wheat, dried alfalfa, soybean meal, sunflower meal, dicalcium phosphate, calcium carbonate and vitamin-mineral premix. The animals from the first experimental group (n = 20; 10♂ and 10♀) were fed a complete mixture added with 1% of nettle (Urtica dioica L.) leaves. Rabbits from the second group (n = 20; 10♂ and 10♀) were fed with a complete mixture added with 1% of fenugreek (Trigonella foenum-graecum L.) seeds. Rabbits fed with pellets with the addition of nettle were characterized by a higher slaughter weight, higher weight of hot and cold carcasses, lungs, kidneys and head as well as a higher weight of the fore, middle and hind part of the carcass compared to the other two groups. The carcasses of animals fed with fenugreek and nettle had a higher percentage of the fore and hind parts compared to the carcasses of the animals from the control group. The female carcasses were characterized by a significantly higher percentage of the middle part compared to the male carcasses. For most colour measurement traits, the differences depending on the feeding regime were significant. The effect of gender on meat colour was non significant. The effect of feeding regime and of gender on texture traits such as shear force, hardness, springiness, cohesiveness and chewiness were non significant. Feeding had no effect on muscle fibre diameter, but it affected the muscle fibre type I percentage. Thus, the group fed with pellets containing nettle leaves had higher percentage of type I muscle fibres than the control group. The effect of gender on muscle fibre traits was non significant. Full article
(This article belongs to the Special Issue Meat Quality and Protein Expression in Livestock and Poultry)
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