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Applied Sciences
Special Issues
Biotechnology of Plants and Pathogens
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Biotechnology of Plants and Pathogens

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Applied Biosciences and Bioengineering".

Deadline for manuscript submissions: closed (20 October 2023) | Viewed by 1034

Special Issue Editors

Dr. Ying Zhai

E-Mail Website
Guest Editor
Department of Plant Pathology, Washington State University, Pullman, WA 99164, USA
Interests: plant virology; plant virus diagnosis; molecular plant–virus interactions; genome editing of plant pathogens; genomics
Special Issues, Collections and Topics in MDPI journals
Dr. Chuntao Yin

E-Mail Website
Guest Editor
Integrated Cropping Systems Research Unit, USDA-ARS, Brookings, SD 57006, USA
Interests: wheat rust diseases; fungal biology; soil microbiomes; crop–pathogen interactions; cropping systems
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Interactions between various pathogens (fungi, bacteria, viruses, nematodes, oomycetes, phytoplasmas, etc.) and their host plants represent an important research field in both fundamental and applied plant sciences. Diverse tools are available for integrated plant disease management, such as pathogen/vector/infected-plant exclusion and/or eradication, cropping system improvement, protection using physical barriers, chemical control using fungicides/bactericides/nematicides, biological control using pathogen-suppressing microbes and other agents, and the development of disease-resistant cultivars. The application of modern biotechnologies provides novel approaches for plant disease control, including, but not limited to, plant disease resistance gene identification, the modification of plants and pathogens using cutting-edge genetic engineering and genome editing techniques, the development of new biocontrol agents for reducing pesticide usage, and microbiomes for improving plant tolerance to biotic/abiotic stress. In this Special Issue, we welcome the submission of original research and review manuscripts which revolve around plant–microbe interaction biotechnologies. Relevant applied topics include research works conducted at molecular, genetic, genomic, field, and population levels. 

Dr. Ying Zhai
Dr. Chuntao Yin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant genome editing
  • plant genetic engineering
  • plant disease resistance
  • plant–pathogen interactions
  • plant disease control
  • biological control
  • microbiomes

Published Papers (1 paper)

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Research

9 pages, 1263 KiB  
Article
Sanitation of Apple Cultivars from AP Phytoplasma and ApMV and ACLSV Viruses Using In Vitro Culture and Cryo-Knife Therapy in Liquid Nitrogen
by Jiří Sedlák, Matěj Semerák and Martina Rejlová
Appl. Sci. 2023, 13(13), 7527; https://0-doi-org.brum.beds.ac.uk/10.3390/app13137527 - 26 Jun 2023
Cited by 1 | Viewed by 857
Abstract
Systemic infections with phytoplasmas and viruses threaten the production of healthy plant material under the fruit species certification system. We tested the possibility of sanitation using in vitro culture and cryotherapy. The starting material of the cultivars Golden Delicious (clones A and B), [...] Read more.
Systemic infections with phytoplasmas and viruses threaten the production of healthy plant material under the fruit species certification system. We tested the possibility of sanitation using in vitro culture and cryotherapy. The starting material of the cultivars Golden Delicious (clones A and B), Virginia Crab, and Panenské zlepšené was taken from in vivo plants that tested positive for apple proliferation phytoplasma. The Táborita cultivar was obtained from already established in vitro cultures that had tested positive for apple proliferation phytoplasma, apple mosaic virus, and apple chlorotic leaf spot virus. Cultivars Golden Delicious A, Virginia Crab, and Panenské zlepšené were sanitated from the phytoplasma in the first step, i.e., by sterilization and a subsequent transfer to in vitro conditions. Golden Delicious B remained infected with the phytoplasma, and both viruses, after the in vitro culture phase and together with Táborita, were subjected to cryotherapy by vitrification. In Golden Delicious B, three out of thirteen initial shoot tips regenerated after a liquid nitrogen treatment. Four mericlones were regenerated from 10 initial cryopreserved shoot tips of Táborita. None of the three pathogens were detected by PCR in the regenerated Golden Delicious B mericlones. On the contrary, in the case of Táborita, infection with all the pathogens was detected after regeneration. The results obtained indicate the potential applicability of in vitro cultivation techniques or, if necessary, subsequent cryopreservation as a method for sanitizing against systemic microbial contamination. However, further research on the relationship between pathogens and specific genotypes is needed. Full article
(This article belongs to the Special Issue Biotechnology of Plants and Pathogens)
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