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Applied Sciences
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New Insights into Food Safety
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New Insights into Food Safety

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Food Science and Technology".

Deadline for manuscript submissions: closed (10 November 2021) | Viewed by 2375

Special Issue Editor

Dr. Yolande T.R. Proroga

E-Mail Website
Guest Editor
Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, Naples, Italy
Interests: microbiology; foods; packaging; viruses
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The main topics of this Issue will be regarding food safety, considered as an instrument to ensure food security and safety, and at the same time, able to protect consumers’ health and quality of life. One of the most important issues in food safety is the presence of bacteria which can compromise the physical, microbiological, and chemical properties of the foods and also represent a danger to human health. The most important bacteria able to alter food quality are Campylobacter, Salmonella, Listeria, Shiga-toxin-producing Escherichia coli, Yersinia, and bacterial toxins from these and other organisms.

Since bacteria can be present at different points of the entire food chain, the following are some of the topics proposed for this Special Issue:

  • Foodborne disease ;
  • Toxin-mediated disease;
  • Alternative methods to improve the detection of food pathogens through the entire food chain;
  • Microbiological characterization and antimicrobial resistance profile of foodborne pathogens;
  • Epidemiological survey of foodborne pathogens.

Dr. Yolande T.R. Proroga
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbiology
  • pathogens
  • antimicrobial resistance
  • innovative food analysis methods
  • food security
  • toxin-mediated disease

Published Papers (1 paper)

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Research

10 pages, 277 KiB  
Article
Droplet Digital PCR (ddPCR) Analysis for Detecting Shiga-Toxin-Producing Escherichia coli (STEC)
by Andrea Mancusi, Andrea Fulgione, Santa Girardi, Orlandina Di Maro, Federico Capuano, Yolande T. R. Proroga and Daniela Cristiano
Appl. Sci. 2022, 12(7), 3654; https://0-doi-org.brum.beds.ac.uk/10.3390/app12073654 - 05 Apr 2022
Cited by 4 | Viewed by 1900
Abstract
Verocytotoxin-producing Escherichia coli, also referred to as Shiga-toxin-producing Escherichia coli (STEC), can be transmitted to humans through person-to-person contact, consumption of contaminated food or water, or by direct contact with animals. Its clinical and economic consequences have prompted the development of alternative [...] Read more.
Verocytotoxin-producing Escherichia coli, also referred to as Shiga-toxin-producing Escherichia coli (STEC), can be transmitted to humans through person-to-person contact, consumption of contaminated food or water, or by direct contact with animals. Its clinical and economic consequences have prompted the development of alternative approaches to the official method of analysis “UNI CEN ISO/TS 13136: 2012”, which describes the identification of STEC through the detection of its main virulence genes. Recently, droplet digital PCR (ddPCR) has been proposed as a technique for the sequence-specific detection and direct quantification of nucleic acids. The present study aimed to investigate if ddPCR could be able to detect STEC in less time than that required by the official method. This study consisted of the ddPCR of slices of beef contaminated with STEC and of the sponges used for beef official control at the slaughter stage. The results showed the ability of ddPCR to detect STEC in slices of beef already after sample incubation for 7 h at 37 °C while, in the case of sponges used for official controls, 9 h at 37 °C was needed. In this way, the ddPCR could represent an efficient method for detecting STEC and providing results in less time than the official method. Full article
(This article belongs to the Special Issue New Insights into Food Safety)
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