Imaging Methods in Cell Biology

Dear Colleagues,

As early as the 17th century, Galileo considered imaging as “the eyes of science”. Nowadays, several imaging techniques provide researchers with a wealth of specific and quantitative imaging information about molecules and cells, and their continuous development and improvement are crucial in disseminating new knowledge in various fields of life sciences. New imaging methods and instrumentation have improved resolution, signal detection, data collection, and manipulation for each sample type, enabling the visualization of a wide range multidimensional and multiparametric data.

The combination of whole-slide imaging at single-cell resolution in spatial biology studies allows for the visualization and quantitative analysis of biomarker expression and co-expression, and multiplexed methods increase the number of disease-specific biomarkers that can be detected simultaneously.

This allows for the dynamic knowledge of biological system components and their respective cellular processes, bridging the gap between morphological and molecular approaches, and can provide a potent tool to diagnose patients, establish the efficacy of therapeutic treatments, and monitor the recurrence of disease. Indeed, the ever-increasing diffusion and precision of imaging tools and the increasingly in-depth knowledge of biological processes allow the development of systems biology models that aim to have real predictive capabilities.

The purpose of this Special Issue is to shed light on the recent advances and development of imaging methods and approaches in different fields of cell biology research.

Prof. Claudia Moscheni
Prof. Nicoletta Gagliano
Guest Editors

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• cellular organelles
• molecular function
• molecular interaction
• disease- and drug-related subcellular phenomena
• single-molecule imaging
• light microscopy
• electron microscopy
• fluorescence microscopy
• fluorescence correlation methods
• fluorescence lifetime imaging microscopy (FLIM)
• Raman microscopy
• spatial biology