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Cells
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Advances in Embryonic Stem Cells and Induced Pluripotent Stem Cells
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Advances in Embryonic Stem Cells and Induced Pluripotent Stem Cells

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: closed (15 August 2023) | Viewed by 3678

Special Issue Editors

Prof. Dr. Douglas Ruden

E-Mail Website
Guest Editor
Institute of Environmental Health Sciences, C. S. Mott Center for Human Growth and Development, Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA
Interests: epigenetics; toxicogenomics; embryonic stem cells; neurotoxicology
Special Issues, Collections and Topics in MDPI journals
Dr. Daniel Rappolee

E-Mail Website
Guest Editor
Department of Ob/Gyn, School of Medicine, Wayne State University, Detroit, MI, USA
Interests: reproductive biology; embryonic stem cells; embryo toxicology

Special Issue Information

Dear Colleagues, 

We are pleased to announce a new Special Issue in Cells titled “Advances in Embryonic Stem Cells.” Embryonic stem cells have been used to identify genes and factors that allow differentiation into numerous other cells types for basic science and regenerative medicine. Embryonic stem cells in combination with trophoblast stem cells have also been combined and differentiated to the gastrulation stage for developmental biology and embryology studies. Embryonic stems cells are also increasingly useful in pharmacology and toxicology studies, both as undifferentiated cells and as highly differentiated organoid models. We welcome all of these types of papers and others that advance the use of embryonic stems cells in the biomedical sciences.

Prof. Dr. Douglas Ruden
Dr. Daniel Rappolee
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • embryonic stem cells
  • trophoblast stem cells
  • toxicology
  • regenerative medicine
  • embryology

Published Papers (1 paper)

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Review

25 pages, 866 KiB  
Review
Epigenetic Reprogramming in Mice and Humans: From Fertilization to Primordial Germ Cell Development
by Aditi Singh, Daniel A. Rappolee and Douglas M. Ruden
Cells 2023, 12(14), 1874; https://0-doi-org.brum.beds.ac.uk/10.3390/cells12141874 - 17 Jul 2023
Cited by 5 | Viewed by 3465
Abstract
In this review, advances in the understanding of epigenetic reprogramming from fertilization to the development of primordial germline cells in a mouse and human embryo are discussed. To gain insights into the molecular underpinnings of various diseases, it is essential to comprehend the [...] Read more.
In this review, advances in the understanding of epigenetic reprogramming from fertilization to the development of primordial germline cells in a mouse and human embryo are discussed. To gain insights into the molecular underpinnings of various diseases, it is essential to comprehend the intricate interplay between genetic, epigenetic, and environmental factors during cellular reprogramming and embryonic differentiation. An increasing range of diseases, including cancer and developmental disorders, have been linked to alterations in DNA methylation and histone modifications. Global epigenetic reprogramming occurs in mammals at two stages: post-fertilization and during the development of primordial germ cells (PGC). Epigenetic reprogramming after fertilization involves rapid demethylation of the paternal genome mediated through active and passive DNA demethylation, and gradual demethylation in the maternal genome through passive DNA demethylation. The de novo DNA methyltransferase enzymes, Dnmt3a and Dnmt3b, restore DNA methylation beginning from the blastocyst stage until the formation of the gastrula, and DNA maintenance methyltransferase, Dnmt1, maintains methylation in the somatic cells. The PGC undergo a second round of global demethylation after allocation during the formative pluripotent stage before gastrulation, where the imprints and the methylation marks on the transposable elements known as retrotransposons, including long interspersed nuclear elements (LINE-1) and intracisternal A-particle (IAP) elements are demethylated as well. Finally, DNA methylation is restored in the PGC at the implantation stage including sex-specific imprints corresponding to the sex of the embryo. This review introduces a novel perspective by uncovering how toxicants and stress stimuli impact the critical period of allocation during formative pluripotency, potentially influencing both the quantity and quality of PGCs. Furthermore, the comprehensive comparison of epigenetic events between mice and humans breaks new ground, empowering researchers to make informed decisions regarding the suitability of mouse models for their experiments. Full article
(This article belongs to the Special Issue Advances in Embryonic Stem Cells and Induced Pluripotent Stem Cells)
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