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Genes
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Application of Transgenic Technology in Animal Breeding
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Application of Transgenic Technology in Animal Breeding

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Transgenic Technology".

Deadline for manuscript submissions: closed (25 April 2022) | Viewed by 7348

Special Issue Editors

Dr. Zhanjun Li

E-Mail Website
Guest Editor
Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China
Interests: transgene animal; animal breeding; pigs; sheep
Dr. Zhiquan Liu

E-Mail Website
Guest Editor
College of Animal Science, Jilin University, Changchun 130015, China
Interests: CRISPR/Cas9; base editor; rabbit; genome editing; AAV
Dr. Tingting Sui

E-Mail Website
Guest Editor
College of Veterinary Medicine, Jilin University, Changchun 130062, China
Interests: disease resistance breeding; transgenic animal breeding; animal model; gene editing

Special Issue Information

Dear Colleagues,

Transgenic technology has been widely used to produce elite livestock breeds, allowing the introduction of alien genes into livestock genomes. In addition, genome-editing technology is a powerful technology that can efficiently alter the genome of organisms to achieve the targeted modification of endogenous genes and the targeted integration of exogenous genes. Current genome-editing tools mainly include ZFN, TALEN and CRISPR/Cas9, which have been applied to various animal species including zebrafish, mice, rabbits, rats, monkeys, pigs, cattle, sheep, goats and others. Transgenic and genome-editing technology can be used in animal breeding for improving disease resistance, carcass composition, lactational performance, wool production, growth rate, and reproductive performance, as well as reducing negative environmental impact. Traditional animal breeding is associated with rate-limiting issues such as long breeding cycle and limitations of genetic resources. Genome-editing tools offer solutions to these problems at affordable costs. The generation of gene-edited animals with improved traits has proven feasible and valuable. Moreover, there are still no genetically engineered animal products on the market for food. It is imperative to comprehensively evaluate the pros and cons they will bring to the animal breeding industry.

For this Special Issue, we will present state-of-the-art applications in transgenic and genome editing technologies in animal breeding, assembling the most recent advances in our field in one place.

Dr. Zhanjun Li
Dr. Zhiquan Liu
Dr. Tingting Sui
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • transgenic
  • genome editing
  • animal breeding
  • CRISPR/Cas9
  • disease resistance

Published Papers (3 papers)

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Research

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11 pages, 2241 KiB  
Article
Efficient Editing of the ZBED6-Binding Site in Intron 3 of IGF2 in a Bovine Model Using the CRISPR/Cas9 System
by Huiying Zou, Dawei Yu, Shun Yao, Fangrong Ding, Junliang Li, Ling Li, Xue Li, Shanjiang Zhao, Yunwei Pang, Haisheng Hao, Weihua Du, Xueming Zhao, Yunping Dai and Huabin Zhu
Genes 2022, 13(7), 1132; https://0-doi-org.brum.beds.ac.uk/10.3390/genes13071132 - 24 Jun 2022
Cited by 3 | Viewed by 2155
Abstract
Background: Insulin-like growth factor 2 is a growth-promoting factor that plays an important role in the growth and development of mammals. A nucleotide substitution in intron 3 of IGF2—which disrupts the ZBED6-binding site—affects muscle mass, organ size, and fat deposition in pigs. [...] Read more.
Background: Insulin-like growth factor 2 is a growth-promoting factor that plays an important role in the growth and development of mammals. A nucleotide substitution in intron 3 of IGF2—which disrupts the ZBED6-binding site—affects muscle mass, organ size, and fat deposition in pigs. The ZBED6-binding site is also conserved in cattle. Methods: In the present study, we introduced mutations in the ZBED6-binding site in intron3 of IGF2 in bovine fetal fibroblasts using the CRISPR/Cas9 system, and investigated the effect of disruption of ZBED6 binding on IGF2 expression. Results: Eleven biallelic-mutant single-cell clones were established, three of which contained no foreign DNA residues. Single-cell clones 93 and 135 were used to produce cloned embryos. Dual-luciferase reporter assay in C2C12 cells demonstrated that the mutation in the ZBED6-binding site increases the promoter 3 activity of bovine IGF2. A total of 49 mutant cloned embryos were transplanted into surrogate cows. Unfortunately, all cloned embryos died before birth. IGF2 was found to be hypomethylated in the only fetus born (stillborn), which may have been due to the incomplete reprogramming. Conclusions: We efficiently constructed IGF2-edited cell lines and cloned embryos, which provided a theoretical basis and experimental materials for beef cattle breeding. Full article
(This article belongs to the Special Issue Application of Transgenic Technology in Animal Breeding)
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Review

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16 pages, 1066 KiB  
Review
Application of CRISPR/Cas Technology in Spermatogenesis Research and Male Infertility Treatment
by Hao-Qi Wang, Tian Wang, Fei Gao and Wen-Zhi Ren
Genes 2022, 13(6), 1000; https://0-doi-org.brum.beds.ac.uk/10.3390/genes13061000 - 1 Jun 2022
Cited by 2 | Viewed by 2603
Abstract
As the basis of animal reproductive activity, normal spermatogenesis directly determines the efficiency of livestock production. An in-depth understanding of spermatogenesis will greatly facilitate animal breeding efforts and male infertility treatment. With the continuous development and application of gene editing technologies, they have [...] Read more.
As the basis of animal reproductive activity, normal spermatogenesis directly determines the efficiency of livestock production. An in-depth understanding of spermatogenesis will greatly facilitate animal breeding efforts and male infertility treatment. With the continuous development and application of gene editing technologies, they have become valuable tools to study the mechanism of spermatogenesis. Gene editing technologies have provided us with a better understanding of the functions and potential mechanisms of action of factors that regulate spermatogenesis. This review summarizes the applications of gene editing technologies, especially CRISPR/Cas9, in deepening our understanding of the function of spermatogenesis-related genes and disease treatment. The problems of gene editing technologies in the field of spermatogenesis research are also discussed. Full article
(This article belongs to the Special Issue Application of Transgenic Technology in Animal Breeding)
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Other

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11 pages, 2034 KiB  
Brief Report
TSA Activates Pluripotency Factors in Porcine Recloned Embryos
by Tao Feng, Xiaolan Qi, Huiying Zou, Shuangyu Ma, Dawei Yu, Fei Gao, Zhengxing Lian, Sen Wu and Xuguang Du
Genes 2022, 13(4), 649; https://0-doi-org.brum.beds.ac.uk/10.3390/genes13040649 - 7 Apr 2022
Cited by 1 | Viewed by 1872
Abstract
Animal cloning is of great importance to the production of transgenic and genome-edited livestock. Especially for multiple gene-editing operations, recloning is one of the most feasible methods for livestock. In addition, a multiple-round cloning method is practically necessary for animal molecular breeding. However, [...] Read more.
Animal cloning is of great importance to the production of transgenic and genome-edited livestock. Especially for multiple gene-editing operations, recloning is one of the most feasible methods for livestock. In addition, a multiple-round cloning method is practically necessary for animal molecular breeding. However, cloning efficiency remains extremely low, especially for serial cloning, which seriously impedes the development of livestock breeding based on genome editing technology. The incomplete reprogramming and failure in oocyte activation of some pluripotent factors were deemed to be the main reason for the low efficiency of animal recloning. Here, to overcome this issue, which occurred frequently in the process of animal recloning, we established a reporter system in which fluorescent proteins were driven by pig OCT4 or SOX2 promoter to monitor the reprogramming process in cloned and recloned pig embryos. We studied the effect of different histone deacetylase (HDAC) inhibitors on incomplete reprogramming. Our results showed that Trichostatin A (TSA) could activate pluripotent factors and significantly enhance the development competence of recloned pig embryos, while the other two inhibitors, valproic acid (VPA) and Scriptaid, had little effect on that. Furthermore, we found no difference in OCT4 mRNA abundance between TSA-treated and untreated embryos. These findings suggest that TSA remarkably improves the reprogramming state of pig recloned embryos by restoring the expression of incompletely activated pluripotent genes OCT4 and SOX2. Full article
(This article belongs to the Special Issue Application of Transgenic Technology in Animal Breeding)
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