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Communication

Functional Characterization of Muscarinic Receptors in Human Schwann Cells

1
Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, Italy
2
Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PT, UK
3
Research Centre of Neurobiology “Daniel Bovet”, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, Italy
4
Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester M23 9LT, UK
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6666; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186666
Received: 23 July 2020 / Revised: 27 August 2020 / Accepted: 9 September 2020 / Published: 11 September 2020
(This article belongs to the Special Issue Neuroprotective Strategies 2020)
Functional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both in central and peripheral nervous system. Rat Schwann cells (SCs) express different muscarinic receptor subtypes with the prevalence of the M2 subtype. The selective stimulation of this receptor subtype inhibits SC proliferation, improving their differentiation towards myelinating phenotype. In this work, we describe for the first time that human SCs are cholinoceptive as they express several muscarinic receptor subtypes and, as for rat SCs, M2 receptor is one of the most abundant. Human SCs, isolated from adult nerves, were cultured in vitro and stimulated with M2 muscarinic agonist arecaidine propargyl ester (APE). Similarly to that observed in rat, M2 receptor activation causes a decreased cell proliferation and promotes SC differentiation as suggested by increased Egr2 expression with an improved spindle-like shape cell morphology. Conversely, the non-selective stimulation of muscarinic receptors appears to promote cell proliferation with a reduction of SC average cell diameter. The data obtained demonstrate that human SCs are cholinoceptive and that human cultured SCs may represent an interesting tool to understand their physiology and increase the knowledge on how the cholinergic stimulation may contribute to address human SC development in normal and pathological conditions. View Full-Text
Keywords: human Schwann cells; acetylcholine; muscarinic receptors; differentiation; glial cells human Schwann cells; acetylcholine; muscarinic receptors; differentiation; glial cells
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MDPI and ACS Style

Piovesana, R.; Faroni, A.; Tata, A.M.; Reid, A.J. Functional Characterization of Muscarinic Receptors in Human Schwann Cells. Int. J. Mol. Sci. 2020, 21, 6666. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186666

AMA Style

Piovesana R, Faroni A, Tata AM, Reid AJ. Functional Characterization of Muscarinic Receptors in Human Schwann Cells. International Journal of Molecular Sciences. 2020; 21(18):6666. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186666

Chicago/Turabian Style

Piovesana, Roberta, Alessandro Faroni, Ada M. Tata, and Adam J. Reid 2020. "Functional Characterization of Muscarinic Receptors in Human Schwann Cells" International Journal of Molecular Sciences 21, no. 18: 6666. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21186666

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