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Article

The Fate of Transplanted Olfactory Progenitors Is Conditioned by the Cell Phenotypes of the Receiver Brain Tissue in Cocultures

1
Inserm UMR1256/NGERE, Faculté de Medecine, 9 Avenue de la Forêt de Haye, Université de Lorraine, F-54000 Nancy, France
2
Laboratoire de Neurosciences, Place Leclerc, Université de Franche-Comté, F-25000 Besancon, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7249; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197249
Received: 5 August 2020 / Revised: 13 September 2020 / Accepted: 25 September 2020 / Published: 30 September 2020
(This article belongs to the Special Issue Neuroregeneration and Brain Repair after Stroke)
Among the numerous candidates for cell therapy of the central nervous system (CNS), olfactory progenitors (OPs) represent an interesting alternative because they are free of ethical concerns, are easy to collect, and allow autologous transplantation. In the present study, we focused on the optimization of neuron production and maturation. It is known that plated OPs respond to various trophic factors, and we also showed that the use of Nerve Growth Factor (NGF) allowed switching from a 60/40 neuron/glia ratio to an 80/20 one. Nevertheless, in order to focus on the integration of OPs in mature neural circuits, we cocultured OPs in primary cultures obtained from the cortex and hippocampus of newborn mice. When dissociated OPs were plated, they differentiated into both glial and neuronal phenotypes, but we obtained a 1.5-fold higher viability in cortex/OP cocultures than in hippocampus/OP ones. The fate of OPs in cocultures was characterized with different markers such as BrdU, Map-2, and Synapsin, indicating a healthy integration. These results suggest that the integration of transplanted OPs might by affected by trophic factors and the environmental conditions/cell phenotypes of the host tissue. Thus, a model of coculture could provide useful information on key cell events for the use of progenitors in cell therapy. View Full-Text
Keywords: cellular therapy; olfactory progenitors; circuit integration; cortex; hippocampus cellular therapy; olfactory progenitors; circuit integration; cortex; hippocampus
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MDPI and ACS Style

Grégory, P.; Nassila, A.; Jean-Louis, M.; Jean-Louis, G.; Jean-Luc, D.; Carine, B.-P. The Fate of Transplanted Olfactory Progenitors Is Conditioned by the Cell Phenotypes of the Receiver Brain Tissue in Cocultures. Int. J. Mol. Sci. 2020, 21, 7249. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197249

AMA Style

Grégory P, Nassila A, Jean-Louis M, Jean-Louis G, Jean-Luc D, Carine B-P. The Fate of Transplanted Olfactory Progenitors Is Conditioned by the Cell Phenotypes of the Receiver Brain Tissue in Cocultures. International Journal of Molecular Sciences. 2020; 21(19):7249. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197249

Chicago/Turabian Style

Grégory, Pourié, Akchiche Nassila, Millot Jean-Louis, Guéant Jean-Louis, Daval Jean-Luc, and Bossenmeyer-Pourié Carine. 2020. "The Fate of Transplanted Olfactory Progenitors Is Conditioned by the Cell Phenotypes of the Receiver Brain Tissue in Cocultures" International Journal of Molecular Sciences 21, no. 19: 7249. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197249

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