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Article

A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction

1
Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Killianstrasse 5, 79106 Freiburg, Germany
2
Department of Ophthalmology, University Hospital Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Schwabachanlage 6, D-91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Emmanuel Stratakis
Int. J. Mol. Sci. 2021, 22(18), 10067; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810067
Received: 10 August 2021 / Revised: 13 September 2021 / Accepted: 14 September 2021 / Published: 17 September 2021
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications)
The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted. A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure. Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs. Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran. We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus. The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo. Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition. In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs. Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue. Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD. View Full-Text
Keywords: decellularization; limbal tissue engineering; limbal transplantation; ex vivo transplantation; recellularization; decellularized limbal tissue; limbal stem cells; limbal melanocytes decellularization; limbal tissue engineering; limbal transplantation; ex vivo transplantation; recellularization; decellularized limbal tissue; limbal stem cells; limbal melanocytes
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MDPI and ACS Style

Polisetti, N.; Roschinski, B.; Schlötzer-Schrehardt, U.; Maier, P.; Schlunck, G.; Reinhard, T. A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction. Int. J. Mol. Sci. 2021, 22, 10067. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810067

AMA Style

Polisetti N, Roschinski B, Schlötzer-Schrehardt U, Maier P, Schlunck G, Reinhard T. A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction. International Journal of Molecular Sciences. 2021; 22(18):10067. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810067

Chicago/Turabian Style

Polisetti, Naresh, Benjamin Roschinski, Ursula Schlötzer-Schrehardt, Philip Maier, Günther Schlunck, and Thomas Reinhard. 2021. "A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction" International Journal of Molecular Sciences 22, no. 18: 10067. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810067

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