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Article

Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators

1
Curriculum in Toxicology and Environmental Medicine, North Carolina Jaycee Burn Center, Department of Surgery, Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
2
Curriculum in Toxicology and Environmental Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
3
Department of Pharmacology, School of Medicine, UNC Proteomics Core Facility, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
4
North Carolina Jaycee Burn Center, Department of Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
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Department of Microbiology and Immunology, Division of Oral and Craniofacial Health Sciences, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
6
Bowles Center for Alcohol Studies, Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Demetrios A. Arvanitis
Int. J. Mol. Sci. 2021, 22(18), 10083; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810083
Received: 22 August 2021 / Revised: 8 September 2021 / Accepted: 14 September 2021 / Published: 18 September 2021
(This article belongs to the Special Issue Extracellular Vesicles as a New Source of Liquid Biopsy 2.0)
Severe burn injury is a devastating form of trauma that results in persistent immune dysfunction with associated morbidity and mortality. The underlying drivers of this immune dysfunction remain elusive, and there are no prognostic markers to identify at-risk patients. Extracellular vesicles (EVs) are emerging as drivers of immune dysfunction as well as biomarkers. We investigated if EVs after burn injury promote macrophage activation and assessed if EV contents can predict length of hospital stay. EVs isolated early from mice that received a 20% total body surface area (TBSA) burn promoted proinflammatory responses in cultured splenic macrophages. Unbiased LC-MS/MS proteomic analysis of early EVs (<72 h post-injury) from mice and humans showed some similarities including enrichment of acute phase response proteins such as CRP and SAA1. Semi-unbiased assessment of early human burn patient EVs found alterations consistent with increased proinflammatory signaling and loss of inhibition of CRP expression. In a sample of 50 patients with large burn injury, EV SAA1 and CRP were correlated with TBSA injury in both sexes and were correlated with length of hospital stay in women. These findings suggest that EVs are drivers of immune responses after burn injury and their content may predict hospital course. View Full-Text
Keywords: biomarkers; extracellular vesicles; burn injury; trauma; sepsis biomarkers; extracellular vesicles; burn injury; trauma; sepsis
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MDPI and ACS Style

Maile, R.; Willis, M.L.; Herring, L.E.; Prevatte, A.; Mahung, C.; Cairns, B.; Wallet, S.; Coleman, L.G., Jr. Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators. Int. J. Mol. Sci. 2021, 22, 10083. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810083

AMA Style

Maile R, Willis ML, Herring LE, Prevatte A, Mahung C, Cairns B, Wallet S, Coleman LG Jr.. Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators. International Journal of Molecular Sciences. 2021; 22(18):10083. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810083

Chicago/Turabian Style

Maile, Robert, Micah L. Willis, Laura E. Herring, Alex Prevatte, Cressida Mahung, Bruce Cairns, Shannon Wallet, and Leon G. Coleman Jr. 2021. "Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators" International Journal of Molecular Sciences 22, no. 18: 10083. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810083

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