Identification, Molecular Characterization, and Tissue Expression Profiles of Three Smad Genes from Water Buffalo (Bubalus bubalis)
Round 1
Reviewer 1 Report
Introduction
I am not convinced that to improve the water buffalo reproductive efficiency the molecular studies are needed. I would argue that your aim should be adjusted in order to support your actual analysis.
LINE 40 - "Gene breeding or genome technology..." - I do not understand this sentence.
Conclusions
I recommend to highlight who your findings will support water buffalo breeding world-wide
Figure 4 should be a heatmap to better present the results.
Figure 6 should be larger
Author Response
Please see the attachment
Author Response File: 
Author Response.docx
Reviewer 2 Report
Dear authors,
Line 1 – “Smads are involved in a variety of biological activities by mediating BMP signals.” – wrong sentence. No descriptions of used abbreviations.
Line 2 – Descript “CDSs” too.
Line 119 – add reference of using software
Line 120 – descript “AASs”
Line 140 – add country information of manufacturer of PCR mix.
Line 155 – isolated by RT-PCR – is it true?
Line 156 – How are you calculated length of amplicons in these gel-frames?
Line 165 – You are can write common color mark for all ABC parts
Line 190 – Text in figure too small. Shorter text mark (only species names) and increase it.
Line 207 – In figure all bootstraps are 0 according to legend. Is it true?
Regards,
Author Response
Please see the attachment.
Author Response File: Author Response.docx
