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Agronomy
Special Issues
Study of Targeted Genome Editing for Crop Improvement
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Study of Targeted Genome Editing for Crop Improvement

A special issue of Agronomy (ISSN 2073-4395). This special issue belongs to the section "Crop Breeding and Genetics".

Deadline for manuscript submissions: closed (31 March 2024) | Viewed by 1949

Special Issue Editors

Dr. Bohan Liu

E-Mail Website
Guest Editor
College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Interests: rice; transcription factor; miRNA; crop improvement; genome editing
Dr. Shuan Meng

E-Mail Website
Co-Guest Editor
College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Interests: plant nutrient; abiotic stress; heavy metal; rice yield and grain quality

Special Issue Information

Dear Colleagues,

With rapid advances in genome editing systems, and ongoing research on plant functional genomics and genome editing techniques, crop improvement and breeding has entered the new era—“Precision breeding”. The precision genome editing systems, such as CRIPSPR/Cas and its derived system (base editing and prime editing), have become an important tool in the hands of breeders to get the desirable trait or new varieties.

In this Special Issue, we welcome research papers and reviews describe the targets, effects, and improvements of targeted crop genome editing for crop improvement. We encourage reports about novel editing methods and target sites for improving disease resistance, abiotic stress tolerance, yield and nutrition utilization efficiency in crops. In particular, developing and migrating novel genome editing systems to crops are highly appreciated in this Special Issue.

Dr. Bohan Liu
Guest Editor

Dr. Shuan Meng
Co-Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Agronomy is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genome editing
  • crop improvement
  • CRISPR/Cas
  • base editor
  • prime editor
  • yield
  • abiotic stress
  • nutrition
  • disease resistance

Published Papers (1 paper)

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Research

22 pages, 4710 KiB  
Article
Genetic Analysis of S5 Regulating the Hybrid Sterility between Indica and Japonica Subspecies in Rice
by Jie Guo, Xinqiao Zhou, Ke Chen, Chanjuan Ye, Juan Liu, Kangli Sun, Guang Tang, Shaokui Wang, Guiquan Zhang, Youding Chen, Dagang Chen and Chuanguang Liu
Agronomy 2023, 13(4), 1094; https://0-doi-org.brum.beds.ac.uk/10.3390/agronomy13041094 - 11 Apr 2023
Viewed by 1522
Abstract
Hybrid sterility is the major obstacle to the utilization of inter-subspecific heterosis in hybrid rice breeding. The S5 locus, composed of three adjacent genes ORF3, ORF4, and ORF5, plays a crucial role in regulating indica/japonica hybrids’ female sterility. [...] Read more.
Hybrid sterility is the major obstacle to the utilization of inter-subspecific heterosis in hybrid rice breeding. The S5 locus, composed of three adjacent genes ORF3, ORF4, and ORF5, plays a crucial role in regulating indica/japonica hybrids’ female sterility. Through a series of crosses involving 38 parents, three alleles of S5, ORF3+ORF4−ORF5n, ORF3+ORF4+ORF5n, and ORF3−/ORF4−/ORF5n, all could be regarded as wide-compatibility alleles, and when crossed with indica or japonica rice, they all showed significantly high fertility. Then, in order to explore the genes’ function, we further knocked out genes by using CRISPR/Cas9-based genome editing. Our results demonstrate that the ORF3+ was not just the protector in the killer-protector system, and knocking out ORF3 of the indica allele seriously affected the rice’s normal development. We observed the concrete enhancing hybrid spikelet fertility from the crosses between the ORF4+ knockout japonica materials with indica varieties. By conducting the comparative RNA-Seq analysis of young spikelets, we found that the ORF4+/ORF4− could modulate the hybrid fertility by affecting the expressions of genes related to the function of the Golgi apparatus. This study indicated that knocking out the ORF4+ of the japonica allele or using the alleles carrying ORF5n would provide effective approaches to overcome indica/japonica hybrid female sterility in rice breeding. Full article
(This article belongs to the Special Issue Study of Targeted Genome Editing for Crop Improvement)
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