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Portable Sensing Platforms for Environmental, Healthy and Food Safety Diagnostics
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Portable Sensing Platforms for Environmental, Healthy and Food Safety Diagnostics

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (31 August 2020) | Viewed by 19292

Special Issue Editor

Prof. Dr. Camelia Bala

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Director Doctoral School of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania
Interests: sensors and biosensors addressing current emerging problems of clinical, food and environmental importance; bioanalytical chemistry focusing on investigations of basic biochemical mechanisms at bio-interfaces; biomimetic materials and artificial receptors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue addresses the current high demand for affordable devices that can be used by local communities in developing regions to improve healthcare, environmental safety, animal health, and food safety. Research in the field of sensors is continually developing and its evolution is focused on the production of sensors for small portable devices to allow fast, accurate, and on-site detection.

Research in this area exploits nanotechnologies, new sensing materials, and new immobilization procedures for bio/receptors to amplify signals and improve their stability. A new generation of sensors with simple configurations and improved analytical performance are being designed in order to provide on the market with hand-held instruments. These portable sensors need to be able to perform measurements at the point-of-need and in the field.

For this Special Issue, authors are invited to submit articles related to the integration of different techniques that have led to the development of portable sensors and biosensors for various applications through selected examples of environmental monitoring, agri-food control, point-of-care testing for patients, detection of biological warfare agents and on-site detection, and diagnosis of plant diseases. Also, articles addressing various microfluidics/electronics/biological design strategies for biomarkers, measurement techniques, sample preparation, fluid-handling techniques, data collection and analysis challenges, and quality specifications for the development of portable biosensors are welcome. Both research papers and review articles will be considered.

Prof. Dr. Camelia Bala
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • portable system
  • point of care
  • microfluidics
  • paper-based sensing
  • multi-detection of analytes

Published Papers (4 papers)

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Research

14 pages, 2986 KiB  
Article
Smartphone-Based Electrochemical Potentiostat Detection System Using PEDOT: PSS/Chitosan/Graphene Modified Screen-Printed Electrodes for Dopamine Detection
by Xiaoyan Shen, Feng Ju, Guicai Li and Lei Ma
Sensors 2020, 20(10), 2781; https://0-doi-org.brum.beds.ac.uk/10.3390/s20102781 - 14 May 2020
Cited by 41 | Viewed by 6115
Abstract
In this work, a smartphone-based electrochemical detection system was designed and developed for rapid and real-time detection of dopamine (DA). The system included a screen-printed electrode (SPE) used as a sensor, a hand-held electrochemical potentiostat and a smart phone with a specially designed [...] Read more.
In this work, a smartphone-based electrochemical detection system was designed and developed for rapid and real-time detection of dopamine (DA). The system included a screen-printed electrode (SPE) used as a sensor, a hand-held electrochemical potentiostat and a smart phone with a specially designed app. During the detection period, the SPEs modified with poly(3,4-ethylenedioxythiophene) (PEDOT), chitosan (CS) and graphene (G) were used to convert and amplify the electrochemical reaction signals. The electrochemical potentiostat was used to generate excitation electrical signals and collect the electrical signals converted from the sensor. The smartphone—connected to the detector via Bluetooth-was used to control the detector for tests, further process the uploaded data, and plot graphs in real time. Experimental results showed that the self-designed sensing system could be employed for highly selective detection of DA in the presence of interfering substances such as ascorbic acid (AA) and uric acid (UA). CV was carried out to characterize the electrochemical properties of the modified SPEs and the electrochemical behaviors of DA on the modified SPEs. Finally, according to the analysis of DPV responses of DA, the system could detect DA with a detection sensitivity of 0.52 ± 0.01 μA/μM and a limit of detection of 0.29 μM in the linear range of DA concentrations from 0.05 to 70 μM. Full article
(This article belongs to the Special Issue Portable Sensing Platforms for Environmental, Healthy and Food Safety Diagnostics)
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14 pages, 4624 KiB  
Article
Portable and Battery-Powered PCR Device for DNA Amplification and Fluorescence Detection
by Junyao Jie, Shiming Hu, Wenwen Liu, Qingquan Wei, Yizheng Huang, Xinxin Yuan, Lufeng Ren, Manqing Tan and Yude Yu
Sensors 2020, 20(9), 2627; https://0-doi-org.brum.beds.ac.uk/10.3390/s20092627 - 05 May 2020
Cited by 21 | Viewed by 5282
Abstract
Polymerase chain reaction (PCR) is a technique for nucleic acid amplification, which has been widely used in molecular biology. Owing to the limitations such as large size, high power consumption, and complicated operation, PCR is only used in hospitals or research institutions. To [...] Read more.
Polymerase chain reaction (PCR) is a technique for nucleic acid amplification, which has been widely used in molecular biology. Owing to the limitations such as large size, high power consumption, and complicated operation, PCR is only used in hospitals or research institutions. To meet the requirements of portable applications, we developed a fast, battery-powered, portable device for PCR amplification and end-point detection. The device consisted of a PCR thermal control system, PCR reaction chip, and fluorescence detection system. The PCR thermal control system was formed by a thermal control chip and external drive circuits. Thin-film heaters and resistance temperature detectors (RTDs) were fabricated on the thermal control chip and were regulated with external drive circuits. The average heating rate was 32 °C/s and the average cooling rate was 7.5 °C/s. The disposable reaction chips were fabricated using a silicon substrate, silicone rubber, and quartz plate. The fluorescence detection system consisted a complementary metal-oxide-semiconductor (CMOS) camera, an LED, and mirror units. The device was driven by a 24 V Li-ion battery. We amplified HPV16E6 genomic DNA using our device and achieved satisfactory results. Full article
(This article belongs to the Special Issue Portable Sensing Platforms for Environmental, Healthy and Food Safety Diagnostics)
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11 pages, 2361 KiB  
Communication
Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers
by Nadezhda A. Byzova, Yuri Yu. Vengerov, Sergey G. Voloshchuk, Anatoly V. Zherdev and Boris B. Dzantiev
Sensors 2019, 19(24), 5494; https://0-doi-org.brum.beds.ac.uk/10.3390/s19245494 - 12 Dec 2019
Cited by 8 | Viewed by 3550
Abstract
The integration of several controlled parameters within a single test system is experiencing increased demand. However, multiplexed test systems typically require complex manufacturing. Here, we describe a multiplexed immunochromatographic assay that incorporates a conventional nitrocellulose membrane, which is used together with microspot printing, [...] Read more.
The integration of several controlled parameters within a single test system is experiencing increased demand. However, multiplexed test systems typically require complex manufacturing. Here, we describe a multiplexed immunochromatographic assay that incorporates a conventional nitrocellulose membrane, which is used together with microspot printing, to construct adjacent microfluidic “tracks” for multiplexed detection. The 1 mm distance between tracks allows for the detection of up to four different analytes. The following reagents are applied in separate zones: (a) gold nanoparticle conjugates with antibodies against each analyte, (b) other antibodies against each analyte, and (c) antispecies antibodies. The immersion of the test strip in the sample initiates the lateral flow, during which reagents of different specificities move along their tracks without track erosion or reagent mixing. An essential advantage of the proposed assay is its extreme rapidity (1–1.5 min compared with 10 min for common test strips). This assay format was applied to the detection of cardiac and inflammatory markers (myoglobin, D-dimer, and C-reactive protein) in human blood, and was characterized by high reproducibility (8%–15% coefficient of variation) with stored working ranges of conventional tests. The universal character of the proposed approach will facilitate its use for various analytes. Full article
(This article belongs to the Special Issue Portable Sensing Platforms for Environmental, Healthy and Food Safety Diagnostics)
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10 pages, 1986 KiB  
Article
A Portable, Single-Use, Paper-Based Microbial Fuel Cell Sensor for Rapid, On-Site Water Quality Monitoring
by Jong Hyun Cho, Yang Gao and Seokheun Choi
Sensors 2019, 19(24), 5452; https://0-doi-org.brum.beds.ac.uk/10.3390/s19245452 - 11 Dec 2019
Cited by 18 | Viewed by 3769
Abstract
Human access to safe water has become a major problem in many parts of the world as increasing human activities continue to spill contaminants into our water systems. To guarantee the protection of the public as well as the environment, a rapid and [...] Read more.
Human access to safe water has become a major problem in many parts of the world as increasing human activities continue to spill contaminants into our water systems. To guarantee the protection of the public as well as the environment, a rapid and sensitive way to detect contaminants is required. In this work, a paper-based microbial fuel cell was developed to act as a portable, single-use, on-site water quality sensor. The sensor was fabricated by combining two layers of paper for a simple, low-cost, and disposable design. To facilitate the use of the sensor for on-site applications, the bacterial cells were pre-inoculated onto the device by air-drying. To eliminate any variations, the voltage generated by the microorganism before and after the air-drying process was measured and calculated as an inhibition ratio. Upon the addition of different formaldehyde concentrations (0%, 0.001%, 0.005%, and 0.02%), the inhibition ratios obtained were 5.9 ± 0.7%, 6.9 ± 0.7%, 8.2 ± 0.6%, and 10.6 ± 0.2%, respectively. The inhibition ratio showed a good linearity with the formaldehyde concentrations at R2 = 0.931. Our new sensor holds great promise in monitoring water quality as a portable, low-cost, and on-site sensor. Full article
(This article belongs to the Special Issue Portable Sensing Platforms for Environmental, Healthy and Food Safety Diagnostics)
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