Increased ROS Scavenging and Antioxidant Efficiency of Chlorogenic Acid Compound Delivered via a Chitosan Nanoparticulate System for Efficient In Vitro Visualization and Accumulation in Human Renal Adenocarcinoma Cells
, Mohd Zobir Hussein 3, Sharida Fakurazi 4, Khatijah Yusoff 5 and Mas Jaffri Masarudin 1,2,3,*Round 1
Reviewer 1 Report
Increased ROS scavenging and antioxidant efficiency of chlorogenic acid compound delivered via a chitosan nanoparticulate system for efficient in vitro visualization and accumulation in human renal adenocarcinoma cells
In the present work " Increased ROS scavenging and antioxidant efficiency of chlorogenic acid compound delivered via a chitosan nanoparticulate system for efficient in vitro visualization and accumulation in human renal adenocarcinoma cells " in this study, a stable, monodispersed, non-toxic CNP synthesized via ionic gelation method at an optimum parameter (600 µL of 0.5 mg/mL of chitosan and 200 µL of 0.7 mg/mL of tripolyphosphate), denoted as CNP°, was used to encapsulate CGA. Sequence of physicochemical analyses and morphological studies were performed to discern the successful formation of the CNP°-CGA hybrid. Antioxidant property in vitro antiproliferative activity of CNP°-CGA, and in vitro accumulation of fluorescently labeled CNP°-CGA in cancer cells were evaluated.
Findings revealed that successful formation of CNP°-CGA hybrid was reveled through an increase in particle size 134.44 ± 18.29 nm (polydispersity index (PDI) 0.29 ± 0.03) as compared to empty CNP°, 80.89 ± 5.16 nm (PDI 0.26 ± 0.01) with a maximal of 12.04 µM CGA loaded per unit weight of CNP° using 20 µM of CGA. This result correlated with Fourier Transform Infrared (FTIR) spectroscopic analysis, transmission (TEM) and field emission scanning (FESEM) electron microscopy, and ImageJ evaluation.
The manuscript is well written and results are well discussed. The authors have been presented an interesting topic and describes clearly the experimental methods and results. Moreover, the paper also uses a good English. Therefore, it may recommended for publication after some modifications:
Results, section 2. Page 11, line 307 . Please add the reference “Chlorogenic acid/PEG-based organic-inorganic hybrids: A versatile sol-gel synthesis route for new bioactive materials , MATERIAL SCIENCE and ENGENEERING C 100 (2019) 837-844
Author Response
Response to Reviewer 1 Comments
Point 1: Results, section 2. Page 11, line 307 . Please add the reference “Chlorogenic acid/PEG-based organic-inorganic hybrids: A versatile sol-gel synthesis route for new bioactive materials , MATERIAL SCIENCE and ENGENEERING C 100 (2019) 837-844
Response 1: I have added the suggested citation #123
Reviewer 2 Report
The authors investigated the development of chitosan nanoparticles encapsulating chlorogenic acid for in vitro visualization and accumulation in human renal cancer cells. From the results of this study, the authors showed the proof-of-concept for the intracellular accumulation, antiproliferative activity, and antioxidant properties of chitosan nanoparticles. Thus, it will be suited for this publication with some minor concern:
In Figure 2, some of results (error bars) was cut-off from the figures. The authors must correct them. In Figure11, the authors must insert scale bar.
Author Response
Point 1: In Figure 2, some of results (error bars) was cut-off from the figures. The authors must correct them. In Figure11, the authors must insert scale bar.
Response 1: I have adjusted the error bars and included scale bar in Figure 11
