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Article

Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production

1
Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
2
OBT Research Center, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
3
Department of Dental Anatomy, Udayan Dental College, House No: 1, Ward No: 7, Chondipur, GPO, Rajpara, Rajshahi 6000, Bangladesh
4
Department of Oral-Maxillofacial Surgery, Dentistry and Orthodontics, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Yuji Takeda
Int. J. Mol. Sci. 2021, 22(17), 9475; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179475
Received: 23 July 2021 / Revised: 27 August 2021 / Accepted: 30 August 2021 / Published: 31 August 2021
(This article belongs to the Special Issue The Role of Interleukin in Health and Diseases)
Tumor-associated macrophages (TAMs) promote cancer cell proliferation and metastasis, as well as anti-tumor immune suppression. Recent studies have shown that tumors enhance the recruitment and differentiation of TAMs, but the detailed mechanisms have not been clarified. We thus examined the influence of cancer cells on the differentiation of monocytes to TAM subsets, including CD163+, CD204+, and CD206+ cells, in oral squamous cell carcinoma (OSCC) using immunohistochemistry, flow cytometry, and a cytokine array. Furthermore, we investigated the effect of OSCC cells (HSC-2, SQUU-A, and SQUU-B cells) on the differentiation of purified CD14+ cells to TAM subsets. The localization patterns of CD163+, CD204+, and CD206+ in OSCC sections were quite different. The expression of CD206 on CD14+ cells was significantly increased after the co-culture with OSCC cell lines, while the expressions of CD163 and CD204 on CD14+ cells showed no change. High concentrations of plasminogen activator inhibitor-1 (PAI-1) and interleukin-8 (IL-8) were detected in the conditioned medium of OSCC cell lines. PAI-1 and IL-8 stimulated CD14+ cells to express CD206. Moreover, there were positive correlations among the numbers of CD206+, PAI-1+, and IL-8+ cells in OSCC sections. These results suggest that PAI-1 and IL-8 produced by OSCC contribute to the differentiation of monocytes to CD206+ TAMs. View Full-Text
Keywords: tumor-associated macrophage; oral squamous cell carcinoma; CD206; plasminogen activator inhibitor–1; interleukin-8 tumor-associated macrophage; oral squamous cell carcinoma; CD206; plasminogen activator inhibitor–1; interleukin-8
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MDPI and ACS Style

Kai, K.; Moriyama, M.; Haque, A.S.M.R.; Hattori, T.; Chinju, A.; Hu, C.; Kubota, K.; Miyahara, Y.; Kakizoe-Ishiguro, N.; Kawano, S.; Nakamura, S. Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production. Int. J. Mol. Sci. 2021, 22, 9475. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179475

AMA Style

Kai K, Moriyama M, Haque ASMR, Hattori T, Chinju A, Hu C, Kubota K, Miyahara Y, Kakizoe-Ishiguro N, Kawano S, Nakamura S. Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production. International Journal of Molecular Sciences. 2021; 22(17):9475. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179475

Chicago/Turabian Style

Kai, Kazuki, Masafumi Moriyama, A. S.M.R. Haque, Taichi Hattori, Akira Chinju, Chen Hu, Keigo Kubota, Yuka Miyahara, Noriko Kakizoe-Ishiguro, Shintaro Kawano, and Seiji Nakamura. 2021. "Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production" International Journal of Molecular Sciences 22, no. 17: 9475. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179475

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