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Microbial Biotechnology in Food

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 30 July 2024 | Viewed by 756

Special Issue Editor


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Guest Editor
Higiene y Seguridad Alimentaria, Instituto Universitario de Investigación de Carne y Productos Cárnicos (IProCar), Facultad de Veterinaria, Universidad de Extremadura, 10003 Cáceres, Spain
Interests: antifungal compounds; proteomics; mycotoxins; metabolomics; food safety; bioprotective agents
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Special Issue Information

Dear Colleagues,

Microorganisms possess an enormous potential to transform substrates. Nutrient-rich foods are a perfect substrate to sustain microbial growth and be transformed into different ones. Additionally, the inclusion of microorganisms in food is usually well-accepted by consumers, since they are within the clean label tendency.

Thus, the use of these microorganisms could contribute to the development of new foods, increase their safety and enhance their physicochemical and sensory properties, as well as their nutritional composition. Finally, the ability of microorganisms to positively impact human health, such as the pre-, pro- and postbiotic activities, deserves to be studied.

Therefore, this Special Issue will focus on the research of the molecular mechanisms and application of microbial biotechnology in food.

Dr. Josué Delgado Perón
Guest Editor

Manuscript Submission Information

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Keywords

  • microbial biotechnology
  • nutrients
  • food
  • molecular mechanisms

Published Papers (1 paper)

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Research

17 pages, 4938 KiB  
Article
The Role of Glucose-6-phosphate Dehydrogenase in the Wine Yeast Hanseniaspora uvarum
by Jürgen J. Heinisch, Andrea Murra, Lucía Fernández Murillo and Hans-Peter Schmitz
Int. J. Mol. Sci. 2024, 25(4), 2395; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms25042395 - 18 Feb 2024
Cited by 1 | Viewed by 639
Abstract
Hanseniaspora uvarum is the predominant yeast species in the majority of wine fermentations, which has only recently become amenable to directed genetic manipulation. The genetics and metabolism of H. uvarum have been poorly studied as compared to other yeasts of biotechnological importance. This [...] Read more.
Hanseniaspora uvarum is the predominant yeast species in the majority of wine fermentations, which has only recently become amenable to directed genetic manipulation. The genetics and metabolism of H. uvarum have been poorly studied as compared to other yeasts of biotechnological importance. This work describes the construction and characterization of homozygous deletion mutants in the HuZWF1 gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), which provides the entrance into the oxidative part of the pentose phosphate pathway (PPP) and serves as a major source of NADPH for anabolic reactions and oxidative stress response. Huzwf1 deletion mutants grow more slowly on glucose medium than wild-type and are hypersensitive both to hydrogen peroxide and potassium bisulfite, indicating that G6PDH activity is required to cope with these stresses. The mutant also requires methionine for growth. Enzyme activity can be restored by the expression of heterologous G6PDH genes from other yeasts and humans under the control of a strong endogenous promoter. These findings provide the basis for a better adaptation of H. uvarum to conditions used in wine fermentations, as well as its use for other biotechnological purposes and as an expression organism for studying G6PDH functions in patients with hemolytic anemia. Full article
(This article belongs to the Special Issue Microbial Biotechnology in Food)
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