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Musculoskeletal Disease: From Molecular Basis to Therapy

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 30 April 2024 | Viewed by 617

Special Issue Editor

Special Issue Information

Dear Colleagues,

Musculoskeletal diseases comprise a diverse range of bone, joint, muscle and connective tissue pathologies and affect all genders, races, and ethnicities. The treatment approaches available for musculoskeletal disorders have substantially progressed in the last decades, especially in terms of pain management and tissue regeneration. However, the puzzle of the pathogenetic mechanisms has not yet been solved, although significant steps have been made, especially in the -omics era, and these developments are a means to fully understand pathobiology with the purpose of producing effective therapeutics.

This Special Issue of IJMS, entitled ‘Musculoskeletal Disease: From Molecular Basis to Therapy’, is led by Dr. Ioannis Kanakis and aims to focus on new insights, novel developments and discoveries, current challenges, and future perspectives in the field of musculoskeletal diseases.

Dr. Ioannis Kanakis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

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Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • musculoskeletal diseases
  • osteoporosis
  • osteoarthritis
  • sarcopenia
  • molecular mechanisms

Published Papers (1 paper)

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Research

16 pages, 39589 KiB  
Article
Defining the Most Potent Osteoinductive Culture Conditions for MC3T3-E1 Cells Reveals No Implication of Oxidative Stress or Energy Metabolism
by Alexandra Semicheva, Ufuk Ersoy, Aphrodite Vasilaki, Ioanna Myrtziou and Ioannis Kanakis
Int. J. Mol. Sci. 2024, 25(8), 4180; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms25084180 - 10 Apr 2024
Viewed by 378
Abstract
The MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge, and, particularly, the osteogenic medium (OSM)’s composition varies in research studies. Therefore, we aimed to [...] Read more.
The MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge, and, particularly, the osteogenic medium (OSM)’s composition varies in research studies. Therefore, we aimed to define the ideal culture conditions for MC3T3-E1 subclone 4 cells with regard to their mineralization capacity and explore if oxidative stress or the cellular metabolism processes are implicated. Cells were treated with nine different combinations of long-lasting ascorbate (Asc) and β-glycerophosphate (βGP), and osteogenesis/calcification was evaluated at three different time-points by qPCR, Western blotting, and bone nodule staining. Key molecules of the oxidative and metabolic pathways were also assessed. It was found that sufficient mineral deposition was achieved only in the 150 μg.mL−1/2 mM Asc/βGP combination on day 21 in OSM, and this was supported by Runx2, Alpl, Bglap, and Col1a1 expression level increases. NOX2 and SOD2 as well as PGC1α and Tfam were also monitored as indicators of redox and metabolic processes, respectively, where no differences were observed. Elevation in OCN protein levels and ALP activity showed that mineralisation comes as a result of these differences. This work defines the most appropriate culture conditions for MC3T3-E1 cells and could be used by other research laboratories in this field. Full article
(This article belongs to the Special Issue Musculoskeletal Disease: From Molecular Basis to Therapy)
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