Utilizing Induced Pluripotent Stem Cells and Cardiomyocytes: From Basic Research to Disease Modeling

Dear Colleagues,

At the molecular level, stem cell research has unlocked new avenues for understanding and manipulating the intricate networks within the heart.

The advent of human induced pluripotent stem cells (iPSCs) and their derivative cardiomyocytes (iPSC-CMs) has allowed for the detailed investigation and manipulation of cardiomyocytes at the molecular level.

At the molecular level, iPSCs undergo a reprogramming process that involves alterations in gene expression patterns, the activation of signaling pathways and epigenetic modifications. This reprogramming converts mature somatic cells into pluripotent stem cells, which can then differentiate into the three germ layers, and consequently to any cell type in the body. The molecular mechanisms underlying this reprogramming process are actively being investigated, with the aim of improving its efficiency and safety for therapeutic applications.

iPSC-CMs behave like native cardiomyocytes, respecting molecular and functional aspects such as the excitation–contraction–coupling machinery and responsiveness to autonomic hormones and neurotransmitters and to a variety of pharmacological agents. Further, iPSC-CMs can be employed to model both acquired and inherited cardiac diseases, as well as screen for novel potential therapeutic modalities. Further, by manipulating the genetic makeup of iPSC-CMs, researchers can gain insights into the molecular basis of inherited cardiac disorders. Additionally, iPSC-CMs can be exposed to various pathological conditions, such as hypoxia or oxidative stress, and to study the molecular responses that occur during heart disease development.

This Special Issue dedicated to iPSC-CMs highlights the latest advancements in basic research, disease modeling and drug development at the molecular level. This Special Issue features technological innovations such as genome editing tools, high-throughput sequencing methods, and single-cell sequencing techniques that have enabled researchers to delve deeper into the molecular mechanisms underlying cardiac diseases. Experimental methods optimized for the study of iPSC-CMs are also discussed, along with their translational potential in clinical settings.

By providing a platform for sharing cutting-edge research and fostering collaborations among the global community of scientists and researchers, we aim to accelerate the development of innovative therapies for cardiovascular diseases. Through collective efforts and the cross-fertilization of ideas, we hope to overcome the challenges in stem cell research and translate the molecular insights gained into effective treatments for patients with cardiac disorders.

Prof. Dr. Ofer Binah
Guest Editor

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• induced pluripotent stem cells
• cardiomyocytes
• disease modeling
• basic research
• translational applications