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Targeting Corneal Wound Healing: Molecular Access to Diagnosis, Pathophysiology, and Treatment

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 20 September 2024 | Viewed by 1047

Special Issue Editor

Special Issue Information

Dear Colleagues,

The wound healing of the cornea decides over the optical visual performance of the eye after incidents. After injury, a quick and complete recovery is desired, albeit after laser surgery a smooth non-regenerative tissue adaption is the target, providing the desired optically perfect outcome without relapse of refractive imperfection. The aim of corneal decides over how corneal wound healing is launched, processed, directed, and finally, stopped. We have come a long way since the initial detection of extracellular matrix components (such as fibronectins, tenascin, etc.), enzymatic activities (such as plasmin), and growth factors (such as EGF, bFGF, etc.) these crucial proteins that were later on called cytokines. Today available at the edge sophisticated techniques, showing unknown precision and diagnostic accuracy have revealed many this far hidden paths of cellular behaviour and control, each offering a new window of understanding and potential therapy – down to the very molecular level. This special issue on this timely so very active area is intended to offer you a platform to present your expertise and data to others, contributing to the progress in the field and a better future for our patients.

Dr. Gysbert van Setten
Guest Editor

Manuscript Submission Information

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Keywords

  • corneal wound healing
  • optical visual performance
  • cornea
  • extracellular matrix
  • enzymatic activities
  • growth factors
  • cytokines

Published Papers (1 paper)

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Research

12 pages, 1869 KiB  
Article
Level of Secretion and the Role of the Nerve Growth Factor in Patients with Keratoconus before and after Collagen Fibre Cross-Linking Surgery
by Magdalena Krok, Ewa Wróblewska-Czajka, Olga Łach-Wojnarowicz, Joanna Bronikowska, Zenon P. Czuba, Edward Wylęgała and Dariusz Dobrowolski
Int. J. Mol. Sci. 2024, 25(1), 366; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms25010366 - 27 Dec 2023
Viewed by 719
Abstract
Background: The aim of this study was to analyse the concentration of the nerve growth factor (NGF-β) in patients with keratoconus (KC) who are undergoing collagen fibre cross-linking (CXL) surgery in order to better understand the pathogenesis of this disease and observe the [...] Read more.
Background: The aim of this study was to analyse the concentration of the nerve growth factor (NGF-β) in patients with keratoconus (KC) who are undergoing collagen fibre cross-linking (CXL) surgery in order to better understand the pathogenesis of this disease and observe the molecular changes occurring after the procedure. Among many cytokines, β-NGF seems to play an important role in the healing processes of corneal damage. Therefore, its role in the regenerative process after CXL treatment may affect the course of treatment and its final results. Tear samples from 52 patients were collected in this prospective study. Additionally, the patients also had a number of tests performed, including corneal topography using optical coherence tomography. Flat (K 1), steep (K 2), cylindrical (CYL), and central corneal thickness (CCT) keratometry were assessed. The tear samples were collected, and other tests were performed before the CXL procedure and afterwards, during the 12-month follow-up period. The NGF concentration was measured using the Bio-Plex Magnetic Luminex Assay. Lower levels of NGF-β were detected in the KC patients than in the control group (p < 0.001). The day after the procedure, the NGF-β level was significantly lower (on average by 2.3 pg/mL) (p = 0.037) than before the procedure, after which, the level of the reagent increases, but only in the group with the advanced cone, one month after CXL it was significantly higher (p = 0.047). Regarding the correlation of NGF with topographic measurements, the following were found: NGF-β correlates significantly (p < 0.05) and positively (r > 0) with K1 before the CXL procedure; NGF-β correlates significantly (p < 0.05) and positively (r > 0) with K1 one month after CXL; NGF-β correlates significantly (p < 0.05) and positively (r > 0) with CYL nine months after CXL; and, after twelve months, NGF-β correlates significantly (p < 0.05) and positively (r > 0) with K2 and K1. Corneal sensitivity did not statistically and significantly correlate with the level of NGF-β secretion. Our study suggests that NGF may be crucial in the development and progression of KC as well as in the repair mechanisms after CXL surgery. Further research is needed on the role of NGF and other inflammatory biomarkers for rapid diagnosis and selection of targeted therapy in patients with keratoconus. Full article
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