Next Article in Journal
Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous prpD and malK in Escherichia coli and Its Transglycosylation Application in Production of Rebaudioside
Next Article in Special Issue
Epigenetics of Muscle- and Brain-Specific Expression of KLHL Family Genes
Previous Article in Journal
In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?
Previous Article in Special Issue
DNA Methylation Is Correlated with Gene Expression during Diapause Termination of Early Embryonic Development in the Silkworm (Bombyx mori)
Article

Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus

1
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
2
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou 310021, China
3
The State Key Laboratory Breeding Base for Sustainable Control of Pests and Disease, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(16), 5753; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21165753
Received: 11 May 2020 / Revised: 28 July 2020 / Accepted: 3 August 2020 / Published: 11 August 2020
Rice black-streaked dwarf virus (RBSDV) causes severe yield losses in rice (Oryza sativa L.) in China. Studies have shown that the mechanisms of DNA methylation-mediated plant defense against DNA viruses and RNA viruses are different. However, in rice its function in response to infection of RBSDV, a double-stranded RNA virus, remains unclear. In this study, high-throughput single-base resolution bisulfite sequencing (BS-Seq) was carried out to analyze the distribution pattern and characteristics of cytosine methylation in RBSDV-infected rice. Widespread differences were identified in CG and non-CG contexts between the RBSDV-infected and RBSDV-free rice. We identified a large number of differentially methylated regions (DMRs) along the genome of RBSDV-infected rice. Additionally, the transcriptome sequencing analysis obtained 1119 differentially expressed genes (DEGs). Correlation analysis of DMRs-related genes (DMGs) and DEGs filtered 102 genes with positive correlation and 71 genes with negative correlation between methylation level at promoter regions and gene expression. Key genes associated with maintaining DNA methylation in rice were analyzed by RT-qPCR and indicated that OsDMT702 might be responsible for the global increase of DNA methylation level in rice under RBSDV stress. Our results suggest important roles of rice DNA methylation in response to RBSDV and provide potential target genes for rice antiviral immunity. View Full-Text
Keywords: DNA methylation; rice; RBSDV; differentially methylated genes; gene expression DNA methylation; rice; RBSDV; differentially methylated genes; gene expression
Show Figures

Graphical abstract

MDPI and ACS Style

Li, L.; He, Y.; Zhang, X.; Zhang, H.; Sun, Z.; Li, J.; Hong, G. Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus. Int. J. Mol. Sci. 2020, 21, 5753. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21165753

AMA Style

Li L, He Y, Zhang X, Zhang H, Sun Z, Li J, Hong G. Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus. International Journal of Molecular Sciences. 2020; 21(16):5753. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21165753

Chicago/Turabian Style

Li, Linying, Yuqing He, Xueying Zhang, Hehong Zhang, Zongtao Sun, Junmin Li, and Gaojie Hong. 2020. "Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus" International Journal of Molecular Sciences 21, no. 16: 5753. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21165753

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop