Special Issue "Hormones and Receptors in Sexual Reproduction"
Deadline for manuscript submissions: 28 February 2022.
Interests: reproduction; endocrinology; gonadotropin-hormones; growth; metabolism; fish; GPCR; hypothalamus–pituitary–gonad axis; molecular modeling; molecular docking
Reproduction is one of the most important concepts in biology, providing the continued existence of all species. Reproduction is controlled by the the hypothalamic–pituitary–gonadal (HPG) axis of intercommunicating neural and endocrine systems that function together in the regulation of fertility.
All reproductive processes involve hormones secreted from the anterior pituitary gland, such as the follicle-stimulating hormone (FSH) and luteinizing hormone (LH), prolactin, growth hormone (GH), adrenocorticotropic hormone (ACTH) and thyroid-stimulating hormone (TSH). Major hormones of reproduction, such as LH and FSH, directly control many aspects of gonadal development and function across vertebrates. The gene expression and secretion of pituitary gonadotropins are regulated by a plethora of neuroendocrine factors from the hypothalamus, endocrine hormones from the peripheral organs such as the gonads, and paracrine factors within the pituitary itself. The neuroenocrine control of reproduction is complex and includes Gnrhs, many neuropeptides (agouti-related peptides, cholecytokinin, galanin, isotocin, kisspeptin, neurokinin B, secretoneurine ghrelin, gonadotropin release inhibiting hormone (GnIH), spexin, neuropeptide Y), aminergic (dopamine, neurorpinephrine, serotonin) and amino acid neurotransmitters (γ-aminobutyric acid, glutamate, taurine). All these hormones act via binding to specific receptors that activate a specific signal transduction mechanism which ultimately leads to cell-type-specific responses. Receptor proteins can be classified by their location. Transmembrane receptors include ion-channel-linked receptors and G-protein-coupled receptors. Intracellular receptors are steroid receptors, which include cytoplasmic receptors and nuclear receptors.
Molecular interactions function as the core regulators of the sexual maturation and reproduction. With the development of newer techniques to study, visualize and manipulate the involved hormones and receptors, newer prespectives and possibilities have emerged for us to study. This Special Issue of International Journal of Molecular Sciences, “Hormones and Receptors in Sexual Reproduction”, aims to shed light on recent deveplopments and prespectives of these molecular mechanisms in sexual reproduction, and welcomes submissions of reviews, opinions, research studies and articles concerning relevant topics.
Prof. Berta Levavi-Sivan
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- luteinizing hormone
- follicle-stimulating hormone
- Neurokinin B
- gonadotropin-inhibitory hormone
- reproductive endocrinology
- sexual maturation
- gonadal development
- sexual behaviour
- reproductive hormones
- hormone–receptor complexes
- steroid hormones
- molecular modeling
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Differential regulation of gonadotropins as revealed by transcriptomes of distinct LH and FSH cells of fish pituitary
Authors: Berta Levavi-Sivan
Affiliation: HUJI, Israel
Title: Functional activity of recombinant forms of Amh and synergistic action with Fsh in European sea bass ovary
Authors: Cinta Zapater; Ana Rocha,; Gregorio Molés; Alessia Mascoli; Soledad Ibañez; Silvia Zanuy; Ana Gómez
Affiliation: CSIC, Spain
Abstract: Although anti-Müllerian hormone (AMH) has classically been correlated with the regression of Müllerian ducts in male mammals, involvement of this growth factor in other reproductive processes only recently come to light. Teleost are the only gnathostomes that lack Müllerian ducts despite having amh orthologous genes. In adult teleost gonads, Amh exerts a role in the early stages of germ cell development in both males and females. Mechanisms involving the interaction of Amh with gonadotropin- and growth factor-induced functions have been proposed, but our overall knowledge regarding Amh function in fish gonads remains modest. In this study we report on Amh actions in the European sea bass ovary. Amh and type 2 Amh receptor (Amhr2) are present in granulosa and theca cells of both early and late-vitellogenic follicles and cannot be detected in previtellogenic ovaries. Using the Pichia pastoris system we have produced a recombinant sea bass Amh that is endogenously processed to generate a 12-15 kDa bioactive mature protein. Contrary to previous evidence in lower vertebrates, we show that, in explants of previtellogenic sea bass ovaries, mature Amh has a synergistic effect on steroidogenesis induced by the follicle-stimulating hormone (Fsh), increasing E2 and cyp19a1a levels.
Title: Characteristics of Neurokinin-3 Receptor and its Binding Sites by Mutational Analysis
Authors: Ishwar Atre; Naama Mizrahi; Berta Levavi-Sivan
Affiliation: Hebrew University of Jerusalem
Abstract: Neurokinin B (NKB) has already been established as a key regulator in reproductive processes in fish, but little is known about the structure and functionality of NKB receptors. In the current study, we created in-silico models for the tilapia NKB receptor (tiTac3Ra), identified its putative orthosteric and allosteric binding sites and key residues involved in binding of tilapia NKB, and its functional homologue NKF. Interestingly, we observed no direct peptide interaction with their tilapia homologs F2516.44 and M2897.43 despite studies in humans suggesting their crucial role in NKB binding. Although, Ala mutations on residues F2516.44 and M2897.43 did not influence binding affinity, they significantly affect the stability of tiTac3Ra, and showed significant reduction in the receptor's activity for NKB and NKF. Further in-vitro studies indicated them to be critical to tiNKB/tiNKF-induced receptor activity. We also report the binding of NKB antagonists to tiTac3Ra, and their in-vitro and in-vivo activity on FSH and LH release and sperm production in mature tilapia males. Non-peptide NKB antagonist SB222200, had a strong inhibitory effect on Tac3Ra activation. SB222200 also decreased LH plasma levels, two hours post IP injection, changed sperm volume and the ratios of the different stages along the spermatogenesis in tilapia testes.
Title: transcriptome and proteome of turkeys hen mature oocyte at F1 developmental stage
Authors: Mariola Słowińska
Affiliation: Instytut Rozrodu Zwierząt i Badań Żywności | Institute of Animal Reproduction and Food Research
Abstract: In birds, the oocyte is highly specialized cell equipped for generating and development of the embryo. The zona pellucida (ZP) matrix that surrounds ovulated oocyte, called inner perivitelline layer (IPVL), is involved in sperm-zona interaction and successful fertilization. To identify the key genes and proteins as well as pathways associated with final step of egg coat development, a next-generation sequencing and two dimensional electrophoresis combined with mass spectrometry were used for analysis of mature oocyte at F1 developmental stage. A total of 7,666 genes and 228 proteins were annotated. The functional profiling was performed using Ingenuity Pathway Analysis (IPA). A strong overlap between IPA canonical pathways imputed from transcriptomic and proteomic data was observed. Six subfamilies of ZP genes, ZP1-4, ZPD and ZPAX were identified. IPA analysis revealed that many of the identified genes/proteins were involved in key steps of protein synthesis via eIF2, elF4 and p70S6K signaling pathways and degradation via ubiquitination. Detailed analysis of transcripts classified in estrogen receptor (ESR) signalling indicated presence of ESR1 and ESR2 and a set of estrogen-dependent genes involved in both genomic and non-genomic mechanism of regulation of gene expression by estrogen. Oxidative phosphorylation was found to be a possible source of ATP in the mature bird oocyte and nuclear factor erythroid 2–related factor signaling pathway could be involved in response against oxidative stress. The oocyte-granulosa cell communication by tight, adherens, and gap junctions seems to be essential for the final step of oocyte maturation. Here, we identified the key physiological process involved in the final stage of oocyte maturation and taking part in IPVL preparation for fertilization. The identified genes/proteins can be potentially used to characterize the specific mechanism of bird reproduction.